Sivgin V, Yalcin G, Kucuk A, Sezen S C, Afandiyeva N, Arslan M
Bratisl Lek Listy. 2020;121(2):117-121. doi: 10.4149/BLL_2020_015.
The aim of this study is to demonstrate whether fullerenol C60 protects renal injury in sevoflurane‑administered rats.
Rats (n: 24) were randomly divided into four groups: Control (Group C), Fullerenol C60 (Group F), Sevoflurane (Group S), Fullerenol C60-Sevoflurane (Group FS). Thirty minutes before the procedure, Fullerenol C60, 100 mg/kg, was administered intraperitoneally. Sevoflurane (2.3 %) was applied for 3 hours to rats in S and FS groups. Biochemical and histopathological parameters were analyzed in renal tissue samples. Kruskal-Wallis and Mann-Whitney U tests were used in statistical analyzes.
Malondialdehyde (MDA) level and catalase (CAT) enzyme activity in Group S were significantly higher than that in all other groups. Paraoxanase (PON) enzyme activity in Group S was significantly lower than in Groups C and FS. The histopathological examination showed that vascular vacuolization and hypertrophy (VVH) and lymphocyte infiltration (LI) were significantly higher in the Group S compared to the Group C.
Renal histopathology revealed that the administration of Fullerenol C60 prior to sevoflurane inhalation reduced oxidative stress and partially corrected the damage caused by anesthesia. We concluded that Fullerenol C60 has a renal protective effect in rats when administered before sevoflurane anesthesia (Tab. 2, Fig. 4, Ref. 40).
本研究旨在证明富勒醇C60是否能保护七氟醚麻醉大鼠的肾损伤。
将24只大鼠随机分为四组：对照组（C组）、富勒醇C60组（F组）、七氟醚组（S组）、富勒醇C60 - 七氟醚组（FS组）。在手术前30分钟，腹腔注射100 mg/kg富勒醇C60。对S组和FS组大鼠应用2.3%的七氟醚3小时。对肾组织样本进行生化和组织病理学参数分析。统计分析采用Kruskal - Wallis检验和Mann - Whitney U检验。
S组丙二醛（MDA）水平和过氧化氢酶（CAT）活性显著高于所有其他组。S组对氧磷酶（PON）活性显著低于C组和FS组。组织病理学检查显示，与C组相比，S组血管空泡化和肥大（VVH）以及淋巴细胞浸润（LI）显著更高。
肾脏组织病理学显示，在吸入七氟醚之前给予富勒醇C60可降低氧化应激，并部分纠正麻醉引起的损伤。我们得出结论，在七氟醚麻醉前给予富勒醇C60对大鼠具有肾保护作用（表2，图4，参考文献40）。
