Department of Anesthesiology and Reanimation, Ankara Training and Research Hospital, Ankara 06230, Turkey.
Department of Anesthesiology and Reanimation, Haymana State Hospital, Ankara 06860, Turkey.
Mol Med Rep. 2024 Mar;29(3). doi: 10.3892/mmr.2024.13178. Epub 2024 Feb 9.
The aim of the present study was to examine the effect of fullerenol C60 on lung and kidney tissue in sevoflurane‑treated rats with lower extremity ischemia‑reperfusion (IR) injury. A total of 30 Wistar albino rats weighing 225‑275 g were used and were equally divided into five groups (n=6/group): i) Sham; ii) IR; iii) IR‑fullerenol C60 (IR‑FUL); iv) IR‑sevoflurane; and v) IR‑fullerenol C60‑sevoflurane (IR‑FUL‑SEVO). Fullerenol C60 was administered intraperitoneally prior to lower extremity IR induction and sevoflurane was administered during the IR injury. Subsequently, lung and kidney histopathological examinations, and serum biochemical analyses were performed. Lung tissue showed markedly increased congestion and neutrophil infiltration in the IR group compared with in the sham group, and notable decreases in congestion and neutrophil infiltration were observed in the treatment groups compared with in the IR group. In the histopathological evaluation of the kidney samples, vacuolization, loss of brush border in tubular epithelial cells, tubular epithelial loss and varying degrees of tubular damage were observed in all groups that underwent IR. There was a significant increase in the mean renal tubule injury score in all IR groups compared with that in the sham group. In addition, the mean kidney injury score was significantly lower in the IR‑FUL and IR‑FUL‑SEVO groups than that in the IR group. It was observed that the expression levels of tumor necrosis factor‑α, interleukin 1β and intercellular adhesion molecule 1 in the lung and kidney tissues were increased following IR, and were decreased in the groups treated with fullerenol C60 and sevoflurane. Notably, it was determined that the reduction in cytokine expression was greatest in the IR‑FUL group. When the oxidant status parameters in the lungs and kidneys were examined, thiobarbituric acid reactive substances levels, and catalase and glutathione S‑transferase enzyme activities were significantly different in the groups receiving sevoflurane or fullerenol C60 treatment compared with those in the IR group. The present study demonstrated the protective effects of fullerenol C60 on the lung and kidney tissues of rats under sevoflurane anesthesia after establishment of lower extremity IR. The results of the present study showed that fullerenol C60 can reduce oxidative and histopathological damage in the lungs and kidneys following IR of the lower extremities.
本研究旨在探讨富勒醇 C60 对七氟醚麻醉下下肢缺血再灌注（IR）损伤大鼠肺和肾组织的影响。使用 30 只体重为 225-275g 的 Wistar 白化大鼠，并将其平均分为五组（n=6/组）：i）假手术；ii）IR；iii）IR-富勒醇 C60（IR-FUL）；iv）IR-七氟醚；和 v）IR-富勒醇 C60-七氟醚（IR-FUL-SEVO）。在下肢 IR 诱导前，通过腹腔内给予富勒醇 C60，在 IR 损伤期间给予七氟醚。随后，进行肺和肾组织病理学检查和血清生化分析。与假手术组相比，IR 组的肺组织显示出明显的充血和中性粒细胞浸润增加，而在治疗组中充血和中性粒细胞浸润明显减少。在肾组织样本的组织病理学评估中，所有接受 IR 的组均观察到空泡化、管状上皮细胞刷状缘丧失、管状上皮细胞丧失和不同程度的管状损伤。与假手术组相比，所有 IR 组的平均肾小管损伤评分均显著增加。此外，与 IR 组相比，IR-FUL 和 IR-FUL-SEVO 组的平均肾脏损伤评分显著降低。观察到 IR 后肺和肾组织中肿瘤坏死因子-α、白细胞介素 1β 和细胞间黏附分子 1 的表达水平增加，而富勒醇 C60 和七氟醚治疗组的表达水平降低。值得注意的是，在 IR-FUL 组中，细胞因子表达的降低最为明显。当检查肺和肾组织中的氧化状态参数时，与 IR 组相比，接受七氟醚或富勒醇 C60 治疗的组的硫代巴比妥酸反应物质水平、过氧化氢酶和谷胱甘肽 S-转移酶酶活性有显著差异。本研究证明了富勒醇 C60 对七氟醚麻醉下建立下肢 IR 后大鼠肺和肾组织的保护作用。本研究结果表明，富勒醇 C60 可减少下肢 IR 后肺和肾组织的氧化和组织病理学损伤。
