Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara 06560, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara 06560, Turkey.
Medicina (Kaunas). 2024 Jul 29;60(8):1232. doi: 10.3390/medicina60081232.
: Lower-extremity ischemia-reperfusion injury can induce distant organ ischemia, and patients with diabetes are particularly susceptible to ischemia-reperfusion injury. Sevoflurane, a widely used halogenated inhalation anesthetic, and fullerenol C60, a potent antioxidant, were investigated for their effects on heart and lung tissues in lower-extremity ischemia-reperfusion injury in streptozotocin (STZ)-induced diabetic mice. : A total of 41 mice were divided into six groups: control ( = 6), diabetes-control ( = 7), diabetes-ischemia ( = 7), diabetes-ischemia-fullerenol C60 ( = 7), diabetes-ischemia-sevoflurane ( = 7), and diabetes-ischemia-fullerenol C60-sevoflurane ( = 7). Diabetes was induced in mice using a single intraperitoneal dose of 55 mg/kg STZ in all groups except for the control group. Mice in the control and diabetes-control groups underwent midline laparotomy and were sacrificed after 120 min. The DIR group underwent 120 min of lower-extremity ischemia followed by 120 min of reperfusion. In the DIR-F group, mice received 100 μg/kg fullerenol C60 intraperitoneally 30 min before IR. In the DIR-S group, sevoflurane and oxygen were administered during the IR procedure. In the DIR-FS group, fullerenol C60 and sevoflurane were administered. Biochemical and histological evaluations were performed on collected heart and lung tissues. : Histological examination of heart tissues showed significantly higher necrosis, polymorphonuclear leukocyte infiltration, edema, and total damage scores in the DIR group compared to controls. These effects were attenuated in fullerenol-treated groups. Lung tissue examination revealed more alveolar wall edema, hemorrhage, vascular congestion, polymorphonuclear leukocyte infiltration, and higher total damage scores in the DIR group compared to controls, with reduced injury parameters in the fullerenol-treated groups. Biochemical analyses indicated significantly higher total oxidative stress, oxidative stress index, and paraoxonase-1 levels in the DIR group compared to the control and diabetic groups. These levels were lower in the fullerenol-treated groups. : Distant organ damage in the lung and heart tissues due to lower-extremity ischemia-reperfusion injury can be significantly reduced by fullerenol C60.
: 下肢缺血再灌注损伤可引起远隔器官缺血，糖尿病患者尤其容易发生缺血再灌注损伤。七氟醚是一种广泛应用的卤代吸入麻醉剂，富勒醇 C60 是一种有效的抗氧化剂，本研究旨在探讨七氟醚和富勒醇 C60 对链脲佐菌素（STZ）诱导的糖尿病小鼠下肢缺血再灌注损伤后心肺组织的影响。 : 共 41 只小鼠分为六组：对照组（n = 6）、糖尿病对照组（n = 7）、糖尿病缺血组（n = 7）、糖尿病缺血富勒醇 C60 组（n = 7）、糖尿病缺血七氟醚组（n = 7）和糖尿病缺血富勒醇 C60 七氟醚组（n = 7）。除对照组外，其余各组小鼠均通过单次腹腔注射 55mg/kg STZ 诱导糖尿病。对照组和糖尿病对照组小鼠行正中开腹术，120min 后处死。DIR 组行 120min 下肢缺血，再灌注 120min。在 DIR-F 组中，IR 前 30min 给予小鼠腹腔注射 100μg/kg 富勒醇 C60。在 DIR-S 组中，IR 过程中给予七氟醚和氧气。在 DIR-FS 组中，给予富勒醇 C60 和七氟醚。收集心、肺组织，行生化和组织学评估。 : 心脏组织的组织学检查显示，与对照组相比，DIR 组的心肌坏死、多形核白细胞浸润、水肿和总损伤评分显著升高，而富勒醇治疗组的这些作用减弱。肺组织检查显示，与对照组相比，DIR 组的肺泡壁水肿、出血、血管充血、多形核白细胞浸润和总损伤评分更高，而富勒醇治疗组的损伤参数减少。生化分析显示，与对照组和糖尿病对照组相比，DIR 组的总氧化应激、氧化应激指数和对氧磷酶 1 水平显著升高，而富勒醇治疗组的这些水平降低。 : 下肢缺血再灌注损伤引起的远隔器官肺和心脏组织损伤可被富勒醇 C60 显著减轻。
