Tural K, Ozden O, Bilgi Z, Kubat E, Ermutlu C S, Merhan O, Findik Guvendi K, Kucuker S A
Bratisl Lek Listy. 2020;121(3):211-217. doi: 10.4149/BLL_2020_032.
Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats.
Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay.
MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. In both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. The numbers of apoptotic cells were significantly decreased in both treatment groups.
Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39).
在各种模型中，甜菜红素和硫酸铜先前已被表明作为抗氧化化合物，对缺血/再灌注（I/R）有益。我们研究了甜菜红素和铜对大鼠心脏和肺的I/R损伤是否具有任何保护作用。
将Spraque-Dawley大鼠分组：假手术组（仅剖腹术）、对照组（仅I/R）、甜菜红素治疗组（在I/R前60分钟腹腔注射（i.p.）100mg/kg甜菜红素）和硫酸铜治疗组（在I/R前7天每天腹腔注射0.1mg/kg硫酸铜）。通过夹闭左肾动脉和主动脉分叉之间的主动脉45分钟诱导缺血。再灌注48小时后，处死大鼠并采集心脏/肺组织。测定丙二醛（MDA）、髓过氧化物酶（MPO）、白细胞介素6（IL-6）水平。通过TUNEL测定法测定细胞凋亡。
I/R组中MDA、MPO、IL-6水平和凋亡细胞显著增加。在两个治疗组中，MDA和MPO水平均降低。甜菜红素给药后，心脏中的IL-6显著降低，但肺中未降低；铜在两个区域均无作用。两个治疗组中的凋亡细胞数量均显著减少。
甜菜红素和铜可能对大鼠心脏和肺的I/R损伤具有保护作用（图6，参考文献39）。
Zotero 插件