Aquatic and Crop Resource Development, National Research Council of Canada, Ottawa, Ontario, Canada.
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
Mol Plant Microbe Interact. 2020 Jun;33(6):842-858. doi: 10.1094/MPMI-11-19-0310-R. Epub 2020 Apr 23.
The mycoparasite ACM941 is under development as a biocontrol organism against , the causative agent of Fusarium head blight in cereals. To identify molecular factors associated with this interaction, the transcriptomic and exometabolomic profiles of and GZ3639 were compared during coculture. Prior to physical contact, the antagonistic activity of correlated with a response heavily dominated by upregulation of polyketide synthase gene clusters, consistent with the detected accumulation of corresponding secondary metabolite products. Similarly, prior to contact, trichothecene gene clusters were upregulated in , while those responsible for fusarielin and fusarin biosynthesis were downregulated, correlating with an accumulation of trichothecene products in the interaction zone over time. A concomitant increase in 15-acetyl deoxynivalenol-3-glucoside in the interaction zone was also detected, with established as the source of this detoxified mycotoxin. After hyphal contact, was found to predominantly transcribe genes encoding cell wall-degradation enzymes, major facilitator superfamily sugar transporters, anion:cation symporters, as well as alternative carbon source utilization pathways, together indicative of a transition to necrotropism at this stage. notably activated the transcription of phosphate starvation pathway signature genes at this time. Overall, a number of signature molecular mechanisms likely contributing to antagonistic activity by against , as well as its mycotoxin tolerance, are identified in this report, yielding several new testable hypotheses toward understanding the basis of as a biocontrol agent for continued agronomic development and application.
生防菌 ACM941 正在被开发为一种针对禾谷镰刀菌的生物防治制剂，禾谷镰刀菌是谷物赤霉病的病原体。为了鉴定与这种相互作用相关的分子因素，在共培养过程中比较了 和 GZ3639 的转录组和外代谢组谱。在物理接触之前， 的拮抗活性与一个主要由聚酮合酶基因簇上调主导的反应相关，与检测到的相应次生代谢产物的积累一致。同样，在接触之前， 中的三萜烯基因簇上调，而负责 fusarielin 和 fusarin 生物合成的基因簇下调，这与随着时间的推移在相互作用区域中三萜烯产物的积累相关。在相互作用区域中还检测到 15-乙酰脱氧雪腐镰刀菌烯醇-3-葡萄糖苷的伴随增加， 被确定为这种解毒真菌毒素的来源。在菌丝接触后， 主要转录编码细胞壁降解酶、主要易化剂超家族糖转运蛋白、阴离子：阳离子转运蛋白以及替代碳源利用途径的基因，这表明在这个阶段向坏死转变。 显著激活了此时磷酸盐饥饿途径特征基因的转录。总的来说，本报告确定了一些可能有助于 对 的拮抗活性及其对真菌毒素的耐受性的特征分子机制，为进一步研究 作为生物防治剂的基础提供了几个新的可测试假说，以促进其在农业发展和应用中的持续发展。
