Henwood Anthony F
Histopathology Department, The Children's Hospital at Westmead , Sydney, Australia.
School of Medicine, University of Western Sydney , Sydney, Australia.
J Histotechnol. 2020 Jun;43(2):102-104. doi: 10.1080/01478885.2020.1734718. Epub 2020 Mar 1.
The 2019 Coronavirus epidemic, provisionally called 2019-nCoV, was first identified in Wuhan, China, in persons exposed to a seafood or wet market. There is an international push to contain the virus and prevent its spread. It is feasible that potentially infectious samples may be received in histopathology laboratories for diagnosis. This technical note presents disinfection procedures and histotechnology processes that should alleviate the risk of infection to laboratory staff. Using data obtained from similar coronaviruses, e.g. severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), experts are confident that 70% ethanol and 0.1% sodium hypochlorite should inactivate the virus. Formalin fixation and heating samples to 56oC, as used in routine tissue processing, were found to inactivate several coronaviruses and it is believed that 2019-nCoV would be similarly affected.
2019年冠状病毒疫情，暂称为2019 - nCoV，最初在中国武汉被发现，患者曾接触过海鲜或湿货市场。国际社会正在努力控制该病毒并防止其传播。有可能会有具有潜在传染性的样本被送到组织病理学实验室进行诊断。本技术说明介绍了消毒程序和组织技术操作流程，这些应能降低实验室工作人员的感染风险。利用从类似冠状病毒（如严重急性呼吸综合征（SARS）和中东呼吸综合征（MERS））获得的数据，专家们确信70%乙醇和0.1%次氯酸钠应能使该病毒失活。常规组织处理中使用的福尔马林固定和将样本加热至56摄氏度被发现可使多种冠状病毒失活，据信2019 - nCoV也会受到类似影响。
