Battelle Memorial Institute, Columbus, Ohio.
U.S. EPA, Durham, North Carolina.
J Occup Environ Hyg. 2022 Aug;19(8):455-468. doi: 10.1080/15459624.2022.2088768. Epub 2022 Jun 28.
The list of EPA-approved disinfectants for coronavirus features many products for use on hard, non-porous materials. There are significantly fewer products registered for use on porous materials. Further, many common, high-touch surfaces fall in between non-porous materials such as glass and porous materials such as soft fabrics. The objective of this study was to assess the efficacy of selected commercially available disinfectant products against coronaviruses on common, high-touch surfaces. Four disinfectants (Clorox Total 360, Bleach solution, Vital Oxide, and Peroxide Multi-Surface Cleaner) were evaluated against Murine Hepatitis Virus A59 (MHV) as a surrogate coronavirus for SARS-CoV-2. MHV in cell culture medium was inoculated onto four materials: stainless steel, latex-painted drywall tape, Styrene Butadiene rubber (rubber), and bus seat fabric. Immediately (T0) or 2-hr (T2) post-inoculation, disinfectants were applied by trigger-pull or electrostatic sprayer and either held for recommended contact times (Spray only) or immediately wiped (Spray and Wipe). Recovered infectious MHV was quantified by median tissue culture infectious dose assay. Bleach solution, Clorox Total 360, and Vital Oxide were all effective (>3-log reduction or complete kill of infectious virus) with both the Spray Only and Spray and Wipe methods on stainless steel, rubber, and painted drywall tape when used at recommended contact times at both T0 and T2 hr. Multi-Surface Cleaner unexpectedly showed limited efficacy against MHV on stainless steel within the recommended contact time; however, it showed increased (2.3 times greater efficacy) when used in the Spray and Wipe method compared to Spray Only. The only products to achieve a 3-log reduction on fabric were Vital Oxide and Clorox Total 360; however, the efficacy of Vital Oxide against MHV on fabric was reduced to below 3-log when applied by an electrostatic sprayer compared to a trigger-pull sprayer. This study highlights the importance of considering the material, product, and application method when developing a disinfection strategy for coronaviruses on high-touch surfaces.
EPA 批准用于冠状病毒的消毒剂清单上有许多用于硬、非多孔材料的产品。用于多孔材料的产品要少得多。此外，许多常见的高接触表面介于非多孔材料（如玻璃）和多孔材料（如软织物）之间。本研究的目的是评估选定的市售消毒剂产品在常见高接触表面对冠状病毒的功效。四种消毒剂（高乐氏全能 360、漂白剂溶液、活力氧化物和多用途双氧水清洁剂）针对作为 SARS-CoV-2 替代物的鼠肝炎病毒 A59（MHV）进行了评估。MHV 在细胞培养液中接种到四种材料上：不锈钢、涂有乳胶的干墙胶带、苯乙烯丁二烯橡胶（橡胶）和公共汽车座椅织物。立即（T0）或接种后 2 小时（T2），通过扳机式或静电喷雾器施用药剂，要么按照推荐的接触时间保持（仅喷雾），要么立即擦拭（喷雾和擦拭）。通过中位数组织培养感染剂量测定法定量回收有感染性的 MHV。漂白剂溶液、高乐氏全能 360 和活力氧化物在 T0 和 T2 小时，在推荐的接触时间内，无论是仅喷雾还是喷雾和擦拭，对不锈钢、橡胶和涂有乳胶的干墙胶带均有效（>3 对数减少或完全杀灭有感染性的病毒）。多用途双氧水清洁剂在推荐的接触时间内对不锈钢表面的 MHV 显示出有限的功效；然而，与仅喷雾相比，在喷雾和擦拭方法中使用时，其功效增加了（2.3 倍）。只有活力氧化物和高乐氏全能 360 能使织物上的 MHV 减少 3 个对数；然而，与扳机式喷雾器相比，静电喷雾器施用于织物时，活力氧化物对 MHV 的功效降低到 3 个对数以下。本研究强调了在制定高接触表面冠状病毒消毒策略时，要考虑材料、产品和应用方法。
