Zhang Mingyue, Ruwe David, Saffari Roja, Kravchenko Mykola, Zhang Weiqi
Department of Psychiatry and Psychotherapy, University of Münster, Münster, Germany.
Front Neurosci. 2020 Feb 7;14:91. doi: 10.3389/fnins.2020.00091. eCollection 2020.
While the transient receptor potential vanilloid 1 (TRPV1) ion channel, a non-selective calcium-permeable cation channel with high Ca permeability, mainly integrates physical and chemical stimuli for nociception, recent studies suggest that it has a role beyond a noxious thermal sensor. In fact, TRPV1 is presently being considered as a target for treating pathophysiological processes including pain, fear, and anxiety disorders. Although this ion channel has many potential roles, its underlying mechanism of action remains elusive. Here we show in mice that activation of TRPV1-, by the exogenous agonist capsaicin-, regulates synaptic activity in both glutamatergic and GABAergic synaptic transmission. Moreover, activation by the endogenous activator N-arachidonoyl taurine (NAT), induced similar effects as capsaicin. On the other hand, taurine, the decomposition product of NAT, strongly depressed the evoked glutamatergic synaptic transmission. In addition to these findings, we also show the immunohistochemical distribution of TRPV1 in the prefrontal cortex (PFC) of mice, as such studies are currently less frequent in the PFC. Overall, these observations allow for a better understanding of how TRPV1 helps regulate excitatory and inhibitory synaptic activity in the PFC of mice.
瞬时受体电位香草酸受体1（TRPV1）离子通道是一种具有高钙通透性的非选择性钙通透阳离子通道，主要整合物理和化学刺激以感受伤害性刺激，然而最近的研究表明它的作用超出了有害热传感器的范畴。事实上，TRPV1目前正被视为治疗包括疼痛、恐惧和焦虑症在内的病理生理过程的靶点。尽管这个离子通道有许多潜在作用，但其潜在的作用机制仍不清楚。在此，我们在小鼠中发现，通过外源性激动剂辣椒素激活TRPV1，可调节谷氨酸能和γ-氨基丁酸能突触传递中的突触活动。此外，内源性激活剂N-花生四烯酰牛磺酸（NAT）激活TRPV1也产生了与辣椒素类似的效果。另一方面，NAT的分解产物牛磺酸强烈抑制诱发的谷氨酸能突触传递。除了这些发现，我们还展示了TRPV1在小鼠前额叶皮质（PFC）中的免疫组织化学分布，因为目前在PFC中这类研究较少。总体而言，这些观察结果有助于更好地理解TRPV1如何帮助调节小鼠PFC中的兴奋性和抑制性突触活动。
