Huang Xing, Song Xiupeng, Chen Rongfa, Zhang Baoqing, Li Changning, Liang Yongsheng, Qiu Lihang, Fan Yegeng, Zhou Zhongfeng, Zhou Huiwen, Lakshmanan Prakash, Li Yangrui, Wu Jianming
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Nanning, China.
Nanning Institute of Agricultural Sciences, Guangxi Academy of Agricultural Science, Nanning, China.
Front Genet. 2020 Feb 5;10:1326. doi: 10.3389/fgene.2019.01326. eCollection 2019.
Drought and cold stresses are the main environmental factors that affect the yield of sugarcane, and genes play very important roles in tolerance to drought, cold, and other environmental stresses. In this study, bioinformatics analysis was performed to characterize genes. RNA sequencing (RNA-seq) was used to detect the expression profiles of induced by cold and drought stresses. According to our results, there are 110 SsDREB subfamily proteins in , which can be classified into six groups; 106 of these genes are distributed among 29 chromosomes. Inter- and intraspecies synteny analyses suggested that all DREB groups have undergone gene duplication, highlighting the polyploid events that played an important role in the expansion of the DREB subfamily. Furthermore, RNA-seq results showed that 45 were up- or downregulated under cold stress; 35 of them were found to be involved in responding to drought stress. According to protein-protein interaction analysis, SsDREB100, SsDREB102, and SsDREB105 play key roles during the response to cold stress. These results reveal that functional divergence exists between collinear homologous genes or among common origin genes in the DREB subfamily of . This study presents a comprehensive analysis and systematic understanding of the precise mechanism of in response to abiotic stress and will lead to improvements in sugarcane.
干旱和低温胁迫是影响甘蔗产量的主要环境因素，而基因在甘蔗对干旱、低温及其他环境胁迫的耐受性中发挥着非常重要的作用。在本研究中，通过生物信息学分析对甘蔗基因进行了表征。利用RNA测序（RNA-seq）检测了甘蔗在低温和干旱胁迫下的基因表达谱。根据我们的结果，甘蔗中有110个SsDREB亚家族蛋白，可分为六组；其中106个基因分布在29条染色体上。种间和种内共线性分析表明，所有DREB组都经历了基因复制，突出了多倍体事件在DREB亚家族扩张中发挥的重要作用。此外，RNA-seq结果显示，45个基因在低温胁迫下上调或下调；其中35个基因被发现参与干旱胁迫响应。根据蛋白质-蛋白质相互作用分析，SsDREB100、SsDREB102和SsDREB105在低温胁迫响应过程中起关键作用。这些结果表明，甘蔗DREB亚家族中共线同源基因或同源起源基因之间存在功能差异。本研究对甘蔗响应非生物胁迫的精确机制进行了全面分析和系统理解，将有助于甘蔗的改良。
