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一种嗜盐草的基因组初步描述。

Initial Description of the Genome of , a Halophile Grass.

作者信息

Hashemi-Petroudi Seyyed Hamidreza, Arab Mozhdeh, Dolatabadi Behnaz, Kuo Yi-Tzu, Baez Mariana Alejandra, Himmelbach Axel, Nematzadeh Ghorbanali, Maibody Seyed Ali Mohammad Mirmohammady, Schmutzer Thomas, Mälzer Michael, Altmann Thomas, Kuhlmann Markus

机构信息

Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

RG Heterosis, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.

出版信息

Front Plant Sci. 2022 Jul 11;13:906462. doi: 10.3389/fpls.2022.906462. eCollection 2022.

Abstract

The use of wild plant species or their halophytic relatives has been considered in plant breeding programs to improve salt and drought tolerance in crop plants. serves as halophyte model for identification and isolation of novel stress adaptation genes. perennial monocot grass, grows in damp or arid areas, often salt-impregnated places and wasteland in cultivated areas, can survive periodically high water salinity, and tolerate high salt concentrations in the soil up to 1,100 mM sodium chloride. Therefore, it serves as valuable genetic resource to understand molecular mechanisms of stress-responses in monocots. The knowledge can potentially be used for improving tolerance to abiotic stresses in economically important crops. Several morphological, anatomical, ecological, and physiological traits of . have been investigated so far. After watering with salt water the grass is able to excrete salt its salt glands. Meanwhile, a number of ESTs (expressed sequence tag), genes and promoters induced by the salt and drought stresses were isolated, sequenced and annotated at a molecular level. Transfer of stress related genes to other species resulted in enhanced stress resistance. Here we describe the genome sequence and structure of analyzed by whole genome sequencing and histological analysis. The chromosome number was determined to be 20 (2 = 2x = 20). The genome size was calculated to be 354 Mb. This genomic information provided here, will support the functional investigation and application of novel genes improving salt stress resistance in crop plants. The utility of the sequence information is exemplified by the analysis of the DREB-transcription factor family.

摘要

在植物育种计划中,人们考虑利用野生植物物种或其盐生近缘种来提高作物对盐和干旱的耐受性。 作为鉴定和分离新型胁迫适应基因的盐生植物模型。 是一种多年生单子叶禾本科植物,生长在潮湿或干旱地区,通常是耕地中的盐碱地和荒地,能周期性地耐受高水盐度,并能耐受土壤中高达1100 mM氯化钠的高盐浓度。因此,它是了解单子叶植物胁迫反应分子机制的宝贵遗传资源。这些知识有可能用于提高经济作物对非生物胁迫的耐受性。目前已经对 的一些形态、解剖、生态和生理特征进行了研究。用盐水浇灌后,这种草能够通过其盐腺排出盐分。同时,在分子水平上分离、测序和注释了一些由盐和干旱胁迫诱导的EST(表达序列标签)、基因和启动子。将胁迫相关基因转移到其他物种中可增强胁迫抗性。在这里,我们描述了通过全基因组测序和组织学分析对 进行分析的基因组序列和结构。确定其染色体数为20(2n = 2x = 20)。计算得出基因组大小为354 Mb。这里提供的这些基因组信息将支持对提高作物耐盐胁迫的新基因的功能研究和应用。DREB转录因子家族的分析例证了序列信息的实用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b6a/9310549/8b5af62d8eea/fpls-13-906462-g001.jpg

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