Liu Ying, Jiang Yaolan, Liu Xiaolan, Cheng Hefen, Han Yuekun, Zhang Dawei, Wu Jinfeng, Liu Lili, Yan Mingli, Que Youxiong, Zhou Dinggang
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, China.
Crop Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410000, China.
Plants (Basel). 2023 Mar 7;12(6):1215. doi: 10.3390/plants12061215.
In plants, the multi-gene family of dual-function hexokinases (HXKs) plays an important role in sugar metabolism and sensing, that affects growth and stress adaptation. Sugarcane is an important sucrose crop and biofuel crop. However, little is known about the gene family in sugarcane. A comprehensive survey of sugarcane , including physicochemical properties, chromosomal distribution, conserved motifs, and gene structure was conducted, identifying 20 members of the gene family that were located on seven of the 32 L. chromosomes. Phylogenetic analysis showed that the family could be divided into three subfamilies (group I, II and III). Motifs and gene structure were related to the classification of . Most contained 8-11 introns which was consistent with other monocots. Duplication event analysis indicated that in L. primarily originated from segmental duplication. We also identified putative cis-elements in the promoter regions which were involved in phytohormone, light and abiotic stress responses (drought, cold et al.). During normal growth and development, 17 were constitutively expressed in all ten tissues. Among them, , and had similar expression patterns and were more highly expressed than other genes at all times. The RNA-seq analysis showed that 14/20 had the highest expression level after cold stress for 6 h, especially , and . As for drought treatment, 7/20 had the highest expression level after drought stress for 10 days, 3/20 (, and ) had the highest expression level after 10 days of recovery. Overall, our results revealed the potential biological function of , which may provide information for in-depth functional verification.
在植物中，双功能己糖激酶（HXK）的多基因家族在糖代谢和糖感知过程中发挥着重要作用，这会影响植物的生长和胁迫适应性。甘蔗是一种重要的蔗糖作物和生物燃料作物。然而，人们对甘蔗中的该基因家族知之甚少。我们对甘蔗进行了全面的研究，包括其理化性质、染色体分布、保守基序和基因结构，鉴定出该基因家族的20个成员，它们位于32条甘蔗染色体中的7条上。系统发育分析表明，该家族可分为三个亚家族（第一组、第二组和第三组）。基序和基因结构与该家族的分类相关。大多数基因含有8 - 11个内含子，这与其他单子叶植物一致。重复事件分析表明，甘蔗中的该基因主要起源于片段重复。我们还在该基因的启动子区域鉴定出了假定的顺式作用元件，这些元件参与植物激素、光和非生物胁迫反应（干旱、寒冷等）。在正常生长发育过程中，17个基因在所有十个组织中组成型表达。其中，ScHXK1、ScHXK2和ScHXK3具有相似的表达模式，并且在所有时期的表达水平都高于其他基因。RNA测序分析表明，20个基因中有14个在冷胁迫6小时后表达水平最高，尤其是ScHXK2、ScHXK3和ScHXK4。至于干旱处理，20个基因中有7个在干旱胁迫10天后表达水平最高，20个基因中有3个（ScHXK1、ScHXK2和ScHXK3）在恢复10天后表达水平最高。总体而言，我们的结果揭示了甘蔗中该基因家族的潜在生物学功能，这可能为深入的功能验证提供信息。
