文献检索，用中文搜 PubMed

Functional microRNA (miRNA) screening for abdominal fat tissue with different dietary vitamin E (VE) levels was performed to reveal miRNAs, genes and metabolic pathways involved in abdominal fat deposition in broilers. 2. A total of 240, one-day-old healthy female chicks were randomly allocated into five dietary treatments containing either 0, 20, 50, 75 or 100 IU DL-α-tocopherol acetate. The sequencing of miRNAs from abdominal fat tissues was performed. The target genes of miRNAs were predicted and enrichment analysis for these genes was performed. Diets supplemented with 50 IU VE significantly diminished abdominal fat deposition in broilers at day 35 of age. 3. A total of 29 miRNAs were differentially expressed between control and 50 IU VE treatment. Ten of the 23 target genes were enriched in four signalling pathways: tight junction, SNARE interactions in vesicular transport, regulation of autophagy and proteasome. 4. This study identified miRNA, target genes and pathways in dietary VE treatment for broilers, providing new insights into the miRNA regulation of abdominal fat deposition in broilers.

为了揭示与肉鸡腹脂沉积相关的 miRNA、基因和代谢途径，我们对不同膳食维生素 E（VE）水平的腹部脂肪组织进行了功能性 microRNA（miRNA）筛选。
240 只 1 日龄健康雌性小鸡被随机分配到 5 种日粮处理组中，分别含有 0、20、50、75 或 100IU DL-α-生育酚乙酸酯。对腹部脂肪组织中的 miRNAs 进行测序。预测了 miRNAs 的靶基因，并对这些基因进行了富集分析。在第 35 天龄时，添加 50IU VE 的日粮显著减少了肉鸡的腹部脂肪沉积。
在对照组和 50IU VE 处理组之间有 29 个 miRNA 存在差异表达。在四个信号通路中，23 个靶基因中的 10 个被富集：紧密连接、囊泡运输中的 SNARE 相互作用、自噬和蛋白酶体的调节。
本研究鉴定了肉鸡日粮 VE 处理中的 miRNA、靶基因和途径，为 miRNA 调控肉鸡腹脂沉积提供了新的见解。

Functional microRNA (miRNA) screening for abdominal fat tissue with different dietary vitamin E (VE) levels was performed to reveal miRNAs, genes and metabolic pathways involved in abdominal fat deposition in broilers. 2. A total of 240, one-day-old healthy female chicks were randomly allocated into five dietary treatments containing either 0, 20, 50, 75 or 100 IU DL-α-tocopherol acetate. The sequencing of miRNAs from abdominal fat tissues was performed. The target genes of miRNAs were predicted and enrichment analysis for these genes was performed. Diets supplemented with 50 IU VE significantly diminished abdominal fat deposition in broilers at day 35 of age. 3. A total of 29 miRNAs were differentially expressed between control and 50 IU VE treatment. Ten of the 23 target genes were enriched in four signalling pathways: tight junction, SNARE interactions in vesicular transport, regulation of autophagy and proteasome. 4. This study identified miRNA, target genes and pathways in dietary VE treatment for broilers, providing new insights into the miRNA regulation of abdominal fat deposition in broilers.

为了揭示与肉鸡腹脂沉积相关的 miRNA、基因和代谢途径，我们对不同膳食维生素 E（VE）水平的腹部脂肪组织进行了功能性 microRNA（miRNA）筛选。
240 只 1 日龄健康雌性小鸡被随机分配到 5 种日粮处理组中，分别含有 0、20、50、75 或 100IU DL-α-生育酚乙酸酯。对腹部脂肪组织中的 miRNAs 进行测序。预测了 miRNAs 的靶基因，并对这些基因进行了富集分析。在第 35 天龄时，添加 50IU VE 的日粮显著减少了肉鸡的腹部脂肪沉积。
在对照组和 50IU VE 处理组之间有 29 个 miRNA 存在差异表达。在四个信号通路中，23 个靶基因中的 10 个被富集：紧密连接、囊泡运输中的 SNARE 相互作用、自噬和蛋白酶体的调节。
本研究鉴定了肉鸡日粮 VE 处理中的 miRNA、靶基因和途径，为 miRNA 调控肉鸡腹脂沉积提供了新的见解。

Suppr 超能文献