Han Guanghong, Liu Weiwei, Jiang Huan, Yu Dongsheng, Hu Min
Department of Oral Geriatrics, Hospital of Stomatology, Jilin University, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling.
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, PR China.
Medicine (Baltimore). 2020 Feb;99(9):e19394. doi: 10.1097/MD.0000000000019394.
This study aimed to investigate the expression of c-Fos and matrix metallopeptidase 9 (MMP-9) in dental pulp of patients receiving orthodontic treatment via wire appliance.Fifteen patients (30 teeth in total) were randomly assigned to five groups: t = 0, t = 1, t = 4, t = 8 and t = 12 (n = 6). The first maxillary premolars of patients in the t = 0 group were extracted without any orthodontic treatment. An intrusive force of 300 g was applied on first maxillary premolars in the other four groups via wire appliances. This force was maintained for 1 week for t = 1 group, 4 weeks for t = 4 group, 8 weeks for t = 8 group, or 12 weeks for t = 12 group, before the teeth were extracted.The expression of c-Fos and MMP-9 in the pulps of each group was analyzed by immunohistochemical staining and real-time PCR. The relationship in the protein expression between c-Fos and MMP-9 in the dental pulp was analyzed by Pearson correlation analysis.Intrusive force of 300 g increased the expression of both c-Fos and MMP-9 in the dental pulp. The protein expression of MMP-9 in the dental pulp was significantly correlated with the expression of c-Fos (P < .001).Extreme intrusive force upregulates c-Fos and MMP-9 expression in the dental pulp. Moreover, protein expression of c-Fos and MMP-9 is significantly correlated under intrusive force.
本研究旨在调查接受钢丝矫治器正畸治疗的患者牙髓中c-Fos和基质金属蛋白酶9（MMP-9）的表达情况。15名患者（共30颗牙齿）被随机分为五组：t = 0、t = 1、t = 4、t = 8和t = 12（n = 6）。t = 0组患者的上颌第一前磨牙未经任何正畸治疗即被拔除。通过钢丝矫治器对其他四组患者的上颌第一前磨牙施加300 g的侵入力。该力在t = 1组维持1周，t = 4组维持4周，t = 8组维持8周，t = 12组维持12周，然后拔牙。通过免疫组织化学染色和实时PCR分析每组牙髓中c-Fos和MMP-9的表达。通过Pearson相关分析分析牙髓中c-Fos和MMP-9蛋白表达之间的关系。300 g的侵入力增加了牙髓中c-Fos和MMP-9的表达。牙髓中MMP-9的蛋白表达与c-Fos的表达显著相关（P <.001）。极端侵入力上调了牙髓中c-Fos和MMP-9的表达。此外，在侵入力作用下，c-Fos和MMP-9的蛋白表达显著相关。
