Badell Edgar, Hennart Mélanie, Rodrigues Carla, Passet Virginie, Dazas Melody, Panunzi Leonardo, Bouchez Valérie, Carmi-Leroy Annick, Toubiana Julie, Brisse Sylvain
Institut Pasteur, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France; National Reference Center for the Corynebacteria of the diphtheriae complex, Paris, France.
Institut Pasteur, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France.
Res Microbiol. 2020 Apr-Jun;171(3-4):122-127. doi: 10.1016/j.resmic.2020.02.003. Epub 2020 Feb 28.
A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF mass spectrometry. The six isolates were negative for the diphtheria toxin gene. Phylogenetic analyses showed that the six isolates (including FRC0190) are clearly demarcated from C. diphtheriae, Corynebacterium belfantii, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. The average nucleotide identity of FRC0190 with C. diphtheriae NCTC11397 was 92.6%, and was 91.8% with C. belfantii FRC0043. C. diphtheriae subsp. lausannense strain CHUV2995 appeared to be a later heterotypic synonym of C. belfantii (ANI, 99.3%). Phenotyping data revealed an atypical negative or heterogeneous intermediate maltose fermentation reaction for the six isolates. MALDI-TOF mass spectrometry differentiated the new group from the other Corynebacterium taxa by the presence of specific spectral peaks. rpoB sequences showed identity to atypical, maltose-negative C. diphtheriae biovar Belfanti isolates previously described from two cats in the USA. We propose the name Corynebacterium rouxii sp. nov. for the novel group, with FRC0190 (= CIP 111752 = DSM 110354) as type strain.
从人类皮肤或腹膜感染以及一只狗身上分离出的一组先前被鉴定为白喉棒状杆菌生物变种贝尔凡蒂（Corynebacterium diphtheriae biovar Belfanti）的六个临床分离株，通过基因组测序、生化分析和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF质谱）进行了特征分析。这六个分离株的白喉毒素基因呈阴性。系统发育分析表明，这六个分离株（包括FRC0190）与白喉棒状杆菌、贝尔凡蒂棒状杆菌（Corynebacterium belfantii）、溃疡棒状杆菌（Corynebacterium ulcerans）和假结核棒状杆菌（Corynebacterium pseudotuberculosis）有明显区分。FRC0190与白喉棒状杆菌NCTC11397的平均核苷酸同一性为92.6%，与贝尔凡蒂棒状杆菌FRC0043的平均核苷酸同一性为91.8%。白喉棒状杆菌劳桑亚种（C. diphtheriae subsp. lausannense）菌株CHUV2995似乎是贝尔凡蒂棒状杆菌的一个后期异型同义词（平均核苷酸同一性，99.3%）。表型数据显示，这六个分离株的麦芽糖发酵反应呈非典型阴性或异质性中间反应。MALDI-TOF质谱通过特定光谱峰的存在将这个新群体与其他棒状杆菌分类群区分开来。rpoB序列与先前在美国从两只猫身上描述的非典型、麦芽糖阴性白喉棒状杆菌生物变种贝尔凡蒂分离株具有同一性。我们提议将这个新群体命名为鲁氏棒状杆菌（Corynebacterium rouxii sp. nov.），以FRC0190（= CIP 111752 = DSM 110354）作为模式菌株。
