Riegel P, Ruimy R, de Briel D, Prévost G, Jehl F, Christen R, Monteil H
Laboratoire de Bactériologie de la Faculté de Médecine, Université Louis-Pasteur, Strasbourg, France.
FEMS Microbiol Lett. 1995 Mar 1;126(3):271-6. doi: 10.1111/j.1574-6968.1995.tb07429.x.
Levels of genomic DNA relatedness were determined using a S1 nuclease procedure for reference bacteria identified as biotypes of Corynebacterium diphtheriae, biovars of Corynebacterium pseudotuberculosis, and 'Corynebacterium ulcerans'. These results showed that the three species are separate taxa at the genomospecies level whereas biotypes and biovars are closely related genomically within each species. Phylogenetic analyses of small-subunit rDNA sequences revealed that 'Corynebacterium ulcerans' forms a tight cluster with Corynebacterium pseudotuberculosis within the robust branch that groups all Corynebacterium sequenced to date. Therefore, we propose that the species incertae sedis 'C. ulcerans' should be conclusively recognized as a distinct species within the genus Corynebacterium with strain CCUG 2708 = NCTC 7910 as type strain. This species is characterized by urease production and fermentation of glycogen.
利用S1核酸酶程序对鉴定为白喉棒状杆菌生物型、伪结核棒状杆菌生物变种和“溃疡棒状杆菌”的参考细菌进行基因组DNA相关性水平测定。结果表明，这三个物种在基因组种水平上是独立的分类单元，而生物型和生物变种在每个物种内基因组上密切相关。小亚基rDNA序列的系统发育分析表明，“溃疡棒状杆菌”与伪结核棒状杆菌在一个紧密的簇中，该簇位于迄今为止所有测序棒状杆菌的稳健分支内。因此，我们建议将待定种“溃疡棒状杆菌”明确认定为棒状杆菌属内的一个独特物种，菌株CCUG 2708 = NCTC 7910作为模式菌株。该物种的特征是产生脲酶和发酵糖原。
