Laboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece.
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, GR-71003, Heraklion, Crete, Greece.
Fish Physiol Biochem. 2020 Jun;46(3):1075-1091. doi: 10.1007/s10695-020-00773-0. Epub 2020 Mar 2.
Μeagre (Argyrosomus regius) is a newly introduced species in the aquaculture sector characterized by high growth rates and good nutritional value. However, the optimum conditions such as water temperature for the rearing of this species are still under research. The aim of the present study was to investigate several biochemical responses in the heart, muscle, liver, and intestine of meagre reared under different water temperatures (17, 23, and 26 °C) in comparison to the initial acclimation temperature at 20 °C. To assess its cellular stress response, the molecular markers of Hsp70 and Hsp90 induced levels, p38 MAPK and p44/42 MAPK phosphorylated levels, ubiquitin conjugates, and Bcl-2 levels were determined. Moreover, to investigate its antioxidant defense, the activities of superoxide dismutase, catalase, and glutathione reductase were measured. The obtained results in meagre showed a distinct tissue-specific response regarding the variations observed in the studied parameters under different acclimation temperatures. Specifically, most examined tissues presented a decrease in the levels of the examined markers compared to 20 °C, while in the intestine, an increase of Hsp70 and p38 MAPK levels was observed at 23 and 26 °C. However, data on the final weight and length of the fish lead to the conclusion that high water temperatures such as 26 °C might be the best for the rearing of this species. Therefore, it is assumed that the observed differential molecular responses may act in a cell-protective way at all temperatures, irrespectively of the effect of temperature on growth parameters.
鲷鱼（Argyrosomus regius）是水产养殖领域新引入的品种，具有生长速度快和营养价值高的特点。然而，其最佳养殖水温等条件仍在研究中。本研究旨在研究不同水温（17、23 和 26°C）下养殖的鲷鱼心脏、肌肉、肝脏和肠道的几种生化反应，并与初始适应温度 20°C 进行比较。为了评估其细胞应激反应，测定了 Hsp70 和 Hsp90 诱导水平、p38 MAPK 和 p44/42 MAPK 磷酸化水平、泛素缀合物和 Bcl-2 水平等分子标记物。此外，为了研究其抗氧化防御能力，测定了超氧化物歧化酶、过氧化氢酶和谷胱甘肽还原酶的活性。研究结果表明，鲷鱼在不同适应温度下观察到的研究参数存在明显的组织特异性反应。具体而言，与 20°C 相比，大多数检查组织的标记物水平下降，而在肠道中，23 和 26°C 时观察到 Hsp70 和 p38 MAPK 水平升高。然而，关于鱼最终体重和长度的数据表明，26°C 等高温可能最适合该品种的养殖。因此，可以假设观察到的差异分子反应可能在所有温度下以细胞保护的方式发挥作用，而与温度对生长参数的影响无关。
