Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100730, China.
Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100730, China.
Endocrine. 2020 May;68(2):448-457. doi: 10.1007/s12020-020-02233-4. Epub 2020 Mar 2.
Insulinoma is a subtype of pancreatic neuroendocrine tumors. Many patients with insulinoma are obese due to frequent food intake. Ghrelin is associated with obesity and blood levels of insulin. It is not clear if plasma levels of ghrelin in insulinoma patients correlate with hyperinsulinemia and obesity. Expression of ghrelin and its receptor has not been well demonstrated in insulinoma.
To study if plasma levels of ghrelin is associated with obesity and hyperinsulinemia or hyperproinsulinemia in patients with insulinoma, and to detect the expression of ghrelin and its receptor in insulinoma.
Plasma levels of acylated ghrelin, insulin, and proinsulin were measured in 37 patients with insulinoma and 25 controls by ELISA. Expression of ghrelin and its receptor GHS-R1A was examined in 20 insulinoma and paired pancreatic specimens by immunostaining. P ≤ 0.05 was considered significant.
The plasma levels of acylated ghrelin in patients with insulinoma were significantly lower than that in the controls (median 15 pg/ml vs. 19 pg/ml, respectively, P = 0.016). The reduced plasma levels of acylated ghrelin in patients were significantly correlated with obesity, hyperinsulinemia, and hyperproinsulinemia (P = 0.029 and P = 0.028, respectively). Expression of ghrelin and its receptor GHS-R1A was shown in the majority of insulinoma specimens. The expression of GHS-R1A was positively correlated with ghrelin expression in insulinoma (P = 0.014).
Plasma levels of acylated ghrelin decreased in patients with insulinoma, probably due to the hyperinsulinemia and obesity in the patients. Expression of both ghrelin and its receptor is common in insulinoma.
胰岛素瘤是胰腺神经内分泌肿瘤的一种亚型。由于频繁进食，许多胰岛素瘤患者肥胖。Ghrelin 与肥胖和胰岛素水平有关。目前尚不清楚胰岛素瘤患者的 Ghrelin 血浆水平是否与高胰岛素血症和肥胖有关。Ghrelin 及其受体的表达在胰岛素瘤中尚未得到很好的证明。
研究胰岛素瘤患者的 Ghrelin 血浆水平是否与肥胖和高胰岛素血症或高胰岛素原血症有关，并检测胰岛素瘤中 Ghrelin 及其受体的表达。
采用 ELISA 法检测 37 例胰岛素瘤患者和 25 例对照者的酰化 Ghrelin、胰岛素和胰岛素原的血浆水平。采用免疫组化法检测 20 例胰岛素瘤及配对胰腺标本中 Ghrelin 及其受体 GHS-R1A 的表达。P≤0.05 为差异有统计学意义。
胰岛素瘤患者的酰化 Ghrelin 血浆水平明显低于对照组（中位数分别为 15pg/ml 和 19pg/ml，P=0.016）。患者酰化 Ghrelin 血浆水平降低与肥胖、高胰岛素血症和高胰岛素原血症显著相关（P=0.029 和 P=0.028）。大多数胰岛素瘤标本中均显示 Ghrelin 和其受体 GHS-R1A 的表达。GHS-R1A 的表达与胰岛素瘤中 Ghrelin 的表达呈正相关（P=0.014）。
胰岛素瘤患者的酰化 Ghrelin 血浆水平降低，可能是由于患者的高胰岛素血症和肥胖所致。Ghrelin 及其受体的表达在胰岛素瘤中很常见。
