Department of Organic Chemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva, Switzerland.
ACS Sens. 2020 Mar 27;5(3):807-813. doi: 10.1021/acssensors.9b02454. Epub 2020 Mar 13.
Detection of specific oligonucleotide sequences is central to numerous applications, and technologies amenable to point-of-care diagnostics or end users are needed. Here, we report a technology making use of a bioluminescent readout and smartphone quantification. The sensor is a semisynthetic luciferase (H-Luc-PNA conjugate) that is turned on by a strand-displacement reaction. We demonstrated sensing of three different microRNAs (miRs), as representative cancer biomarkers, and demonstrate the possibility to integrate an AND gate to sense two sequences simultaneously.
特定寡核苷酸序列的检测是许多应用的核心，因此需要适用于即时诊断或终端用户的技术。在这里，我们报告了一种利用生物发光读出和智能手机定量的技术。该传感器是一种半合成荧光素酶（H-Luc-PNA 缀合物），通过链置换反应被激活。我们演示了对三种不同 microRNAs（miRs）的传感，作为代表性的癌症生物标志物，并展示了集成与门同时检测两个序列的可能性。
