Algama Chamika Harshani, Basir Jamil, Wijesinghe Kalani M, Dhakal Soma
Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284, USA.
Nanomaterials (Basel). 2024 Jul 12;14(14):1185. doi: 10.3390/nano14141185.
The use of DNA structures in creating multimodal logic gates bears high potential for building molecular devices and computation systems. However, due to the complex designs or complicated working principles, the implementation of DNA logic gates within molecular devices and circuits is still quite limited. Here, we designed simple four-way DNA logic gates that can serve as multimodal platforms for simple to complex operations. Using the proximity quenching of the fluorophore-quencher pair in combination with the toehold-mediated strand displacement (TMSD) strategy, we have successfully demonstrated that the fluorescence output, which is a result of gate opening, solely relies on the oligonucleotide(s) input. We further demonstrated that this strategy can be used to create multimodal (tunable displacement initiation sites on the four-way platform) logic gates including YES, AND, OR, and the combinations thereof. The four-way DNA logic gates developed here bear high promise for building biological computers and next-generation smart molecular circuits with biosensing capabilities.
利用DNA结构创建多模态逻辑门在构建分子器件和计算系统方面具有巨大潜力。然而，由于设计复杂或工作原理繁琐，DNA逻辑门在分子器件和电路中的应用仍然相当有限。在此，我们设计了简单的四路DNA逻辑门，可作为从简单操作到复杂操作的多模态平台。通过结合荧光团-猝灭剂对的邻近猝灭与链置换介导的链置换（TMSD）策略，我们成功证明了作为门打开结果的荧光输出仅依赖于寡核苷酸输入。我们进一步证明，该策略可用于创建多模态（四路平台上可调的置换起始位点）逻辑门，包括“是”、“与”、“或”及其组合。本文开发的四路DNA逻辑门在构建具有生物传感能力的生物计算机和下一代智能分子电路方面具有很高的前景。
