Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, USA.
Perspect Med Educ. 2021 Aug;10(4):252-255. doi: 10.1007/s40037-020-00568-2.
There continues to be a disjoint between the emergence of new diagnostic technologies and venues to train new physicians on how to apply them. Next-generation sequencing (NGS) has become a very important tool for a wide range of clinical applications. Technical complexity and cost have been the major obstacles in incorporating these technologies into the classroom.
We opted to use the MinION, which is a new portable DNA sequencer that can produce data in real-time at a relatively low cost, for a NGS hands-on workshop with medical students.
We conducted a pilot NGS hands-on practical module in order to expose an interested group of medical students to this new portable sequencer device. A pre- and post-survey, using a Likert-type scale survey items and open-ended questions, evaluated participant resistance to new diagnostic tools, familiarity with NGS, and likelihood to use a portable sequencer in clinical practice.
Prior to participating in our learning workshop, students did not understand how to incorporate NGS into clinical practice, and expressed that cost and prior training/knowledge were among the limiting factors in their likelihood to use NGS as a diagnostic tool. After participating in the module, students' responses demonstrated a shift in their understanding of the scientific principles and applications of NGS (pre- and post-survey scores p < 0.05).
The hands-on experience not only helped students become closer to and more comfortable with NGS, but also served as a venue to discuss the science and application of this technology in medicine. Such discussion helped to provide participants with current "genetic literacy" that is often incompletely covered in the typical undergraduate medical education curriculum.
新诊断技术的出现与培训新医生如何应用这些技术的场所之间仍然存在脱节。下一代测序（NGS）已成为广泛的临床应用的重要工具。技术复杂性和成本一直是将这些技术纳入课堂的主要障碍。
我们选择使用 MinION，这是一种新的便携式 DNA 测序仪，可以以相对较低的成本实时生成数据，为医学生提供 NGS 实践工作坊。
我们进行了 NGS 实践模块的试点，以使一组有兴趣的医学生接触到这种新的便携式测序仪设备。使用李克特量表的预调查和后调查评估参与者对新诊断工具的抵制程度、对 NGS 的熟悉程度以及在临床实践中使用便携式测序仪的可能性。
在参加我们的学习研讨会之前，学生不了解如何将 NGS 纳入临床实践，并且表示成本和事先的培训/知识是他们使用 NGS 作为诊断工具的可能性的限制因素。参加模块后，学生的反应表明他们对 NGS 的科学原理和应用的理解发生了转变（预调查和后调查得分 p <0.05）。
实践经验不仅帮助学生更接近 NGS 并使其感到更舒适，而且还为讨论该技术在医学中的科学和应用提供了场所。这种讨论有助于为参与者提供当前的“遗传素养”，这在典型的本科医学教育课程中往往没有涵盖。
