Department of Food Quality and Safety, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, PR China.
College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
Int J Biol Macromol. 2020 Jun 1;152:894-903. doi: 10.1016/j.ijbiomac.2020.02.325. Epub 2020 Feb 29.
Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order. Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained. The differences of five GPs in chemical composition, characterization and antitumor activities were further compared. The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa. Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs. While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group. Moreover, UGP1 exhibited strong inhibitory effect on three tumor cells especially the colon cancer. The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively. The study indicated GPs extracted by UCGE could reserve more active structure and inhibit colon cancer more significantly.
采用热水提取（HWE）、酶辅助提取（EAE）和超声细胞研磨提取（UCGE）三种不同的提取技术，在各自的最佳参数下提取粗姜多糖（GPs），然后用 DEAE 纤维素-52 和 Sephadex G-200 大小排阻层析依次对粗 GPs 进行纯化。得到五个 GPs 级分（HGP、EGP1、EGP2、UGP1 和 UGP2）。进一步比较了这五个 GPs 在化学成分、性质和抗肿瘤活性方面的差异。五个 GPs 的分子量不同，从 11.81 到 1831.75 kDa。甘露糖和葡萄糖是主要的单糖，糖苷键为→4）-α-D-Glc（1→和-α-Manp-（1→，存在于五个 GPs 中。而 EGP2 和 UGP1 具有特定的结构→6）-β-D-Galp-（1→和 UGP1 含有更多的硫酸基团。此外，UGP1 对三种肿瘤细胞尤其是结肠癌具有很强的抑制作用。UGP1 对 H1975、HCT116 和 MCF-7 的抑制率分别为 23.339±2.285%、56.843±2.405%和 21.061±1.920%。研究表明，UCGE 提取的 GPs 可以保留更多的活性结构，对结肠癌的抑制作用更为显著。
