Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
Department of Nutrition and Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Environ Res. 2020 May;184:109295. doi: 10.1016/j.envres.2020.109295. Epub 2020 Feb 26.
Phthalate metabolites in follicular fluid (FF) may negatively affect normal folliculogenesis; however, the predictors of phthalate metabolite concentrations in urine and FF and relationships between urine and FF phthalate metabolite concentrations among women undergoing in vitro fertilization (IVF) are poorly understood.
To investigate predictors of phthalate metabolites in urine and FF and correlations between urine and FF phthalate metabolite concentrations among women undergoing IVF.
We recruited 305 women seeking infertility treatment at a reproductive center in Wuhan, China, from October to November 2016. Information regarding demographic characteristics, personal care product use and plastic material contact was obtained through direct interviews. Concentrations of 8 phthalate metabolites in urine and FF samples were measured using high-performance liquid chromatography and tandem mass spectrometry. Associations regarding metabolite concentrations in urine and FF samples were analysed by Spearman's correlation and linear regression. Generalized linear regression was used to examine potential predictors of phthalate metabolite concentrations in urine and FF.
Weak to moderate associations between urine and FF samples were found for monoethyl phthalate (MEP) and mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) (correlation coefficient: MEP, 0.350; MEOHP, 0.377); no associations were observed for other metabolites. The predictive powers of urinary metabolite concentrations in determining FF metabolite concentrations were uniformly low, with R ≤ 0.113. Body mass index (BMI) and educational level were inversely associated with the urinary concentrations of certain metabolites. Higher household income, intake of bottled drinks within 48 h, and use of shower gel and soap were frequently associated with higher urinary metabolite concentrations. BMI, higher household income and use of disposable plastic cups within 48 h were associated with higher metabolite concentrations in FF.
Phthalate metabolite concentrations in urine and FF vary according to sociodemographic characteristics and lifestyle factors. Phthalate metabolite concentrations in urine may not be appropriate for estimating ovary phthalate exposure.
卵泡液（FF）中的邻苯二甲酸代谢物可能会对正常卵泡发生产生负面影响；然而，对于接受体外受精（IVF）的女性尿液和 FF 中邻苯二甲酸代谢物浓度的预测因子以及尿液和 FF 中邻苯二甲酸代谢物浓度之间的关系知之甚少。
探讨接受 IVF 的女性尿液和 FF 中邻苯二甲酸代谢物的预测因子以及尿液和 FF 中邻苯二甲酸代谢物浓度之间的相关性。
我们于 2016 年 10 月至 11 月在中国武汉的一家生殖中心招募了 305 名寻求不孕治疗的女性。通过直接访谈获得了人口统计学特征、个人护理产品使用和塑料材料接触的信息。使用高效液相色谱和串联质谱法测量了尿液和 FF 样本中 8 种邻苯二甲酸代谢物的浓度。通过 Spearman 相关和线性回归分析了尿液和 FF 样本中代谢物浓度的相关性。使用广义线性回归检验了尿液和 FF 中邻苯二甲酸代谢物浓度的潜在预测因子。
发现尿液和 FF 样本中邻苯二甲酸单乙酯（MEP）和邻苯二甲酸单（2-乙基-5-氧代己基）（MEOHP）之间存在弱到中度关联（相关系数：MEP，0.350；MEOHP，0.377）；其他代谢物之间没有关联。尿液代谢物浓度预测 FF 代谢物浓度的能力普遍较低，R ≤ 0.113。体重指数（BMI）和教育水平与某些代谢物的尿液浓度呈负相关。较高的家庭收入、48 小时内摄入瓶装饮料以及使用沐浴露和肥皂与较高的尿液代谢物浓度频繁相关。BMI、较高的家庭收入和 48 小时内使用一次性塑料杯与 FF 中较高的代谢物浓度相关。
尿液和 FF 中的邻苯二甲酸代谢物浓度因社会人口统计学特征和生活方式因素而异。尿液中的邻苯二甲酸代谢物浓度可能不适合估计卵巢邻苯二甲酸暴露。
