Mamnabi Sohrab, Nasrollahzadeh Safar, Ghassemi-Golezani Kazem, Raei Yaghoub
Department of Ecophysiology, Faculty of Agriculture, University of Tabriz, 5166614766 Tabriz, East Azarbayjan, Iran.
Saudi J Biol Sci. 2020 Mar;27(3):797-804. doi: 10.1016/j.sjbs.2020.01.008. Epub 2020 Jan 17.
Two separate field experiments were conducted in 2018 and 2019 as split-plot based on randomized complete block design with three replications to evaluate physiological responses of rapeseed to fertilization treatments (control, chemical fertilizer, inoculation of seeds with PGPR, vermicompost and combined fertilizers) under different irrigation levels (irrigation after 70,100, 130, and 160 mm evaporation). Water stress increased the activities of catalase, polyphenol oxidase, peroxidase and superoxide dismutase and the contents of proline, soluble sugars and malondialdehyde and also leaf temperature, but decreased membrane stability index, chlorophyll content, leaf water content, stomatal conductance and grain yield. Application of fertilizers particularly combined fertilizers decreased proline content and leaf temperature, but increased the antioxidant enzymes activities, soluble sugars, chlorophyll content, leaf water content, membrane stability index, and stomatal conductance under different irrigation intervals. These superiorities of fertilization treatments were led to considerable improvement in grain yield. The results revealed that the combined fertilizer application improved most of the physiological parameters. It was deducted that the application of combined fertilizers reduced chemical fertilizer by about 67% and alleviated the deleterious effects of water limitation on field performance of rapeseed.
2018年和2019年进行了两项独立的田间试验，试验采用基于随机完全区组设计的裂区设计，重复三次，以评估在不同灌溉水平（蒸发70、100、130和160毫米后灌溉）下，油菜对施肥处理（对照、化肥、用植物根际促生菌接种种子、蚯蚓粪和复合肥）的生理响应。水分胁迫增加了过氧化氢酶、多酚氧化酶、过氧化物酶和超氧化物歧化酶的活性以及脯氨酸、可溶性糖和丙二醛的含量，还提高了叶片温度，但降低了膜稳定性指数、叶绿素含量、叶片含水量、气孔导度和籽粒产量。施肥，尤其是复合肥的施用，降低了脯氨酸含量和叶片温度，但在不同灌溉间隔下提高了抗氧化酶活性、可溶性糖、叶绿素含量、叶片含水量、膜稳定性指数和气孔导度。施肥处理的这些优势使籽粒产量有了显著提高。结果表明，复合肥的施用改善了大多数生理参数。据推断，复合肥的施用使化肥用量减少了约67%，并减轻了水分限制对油菜田间表现的有害影响。
