Mubashir Areesha, Nisa Zaib-Un-, Shah Anis Ali, Kiran Munazza, Hussain Iqtidar, Ali Naila, Zhang Lixin, Madnay Mahmoud M Y, Alsiary Waleed A, Korany Shereen Magdy, Ashraf Muhammad, Al-Mur Bandar A, AbdElgawad Hamada
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan.
Front Plant Sci. 2023 Jan 4;13:1066790. doi: 10.3389/fpls.2022.1066790. eCollection 2022.
Drought stress has drastically hampered the growth and yield of many crops. Therefore, environmentally safe agricultural techniques are needed to mitigate drought stress impact. To this end, foliar spray of nano-nutrients solution to (NNS) alleviate harmful aspects of drought stress.
In a completely randomized design (CRD) experiment, seedlings were transplanted into pots at 2-3 leaf stage, each filled with loam-compost- organic manure soil (3:1:1). Plants were divided into two groups. (a) control group (b) applied stress group. Plants at vegetative stage were treated with 100% FC for control group and 60% FC for drought group, and these levels were maintained until harvesting. Three treatments of NNS with four levels i.e., 0%, 1%, 3% and 5% were given to all the pots after two weeks of drought stress treatment with a gap of 5 days at vegetative stage.
Application of 1% of nano-nutrient solution displayed an improvement in shoot length, shoot fresh and dry weight, number of leaves and flowers. Leaf chlorophylls and carotenoids and total phenolics contents were found maximum while minimum electrolyte leakage was observed at 3% application compared to control. Further, 1% application of NNS increased the Leaf RWC%, total soluble sugars, flavonoids contents. 5% NNS application exhibited higher total free amino acids with minimum lipid peroxidation rate in leaves of tomato under drought. Antioxidant enzyme activities increased in a concentration dependent manner as gradual increase was observed at 1%, 3% and 5%, respectively. Overall, this study introduced a new insights on using nano-nutrient solutions to maintain natural resources and ensure agricultural sustainability.
干旱胁迫严重阻碍了许多作物的生长和产量。因此，需要采用环境安全的农业技术来减轻干旱胁迫的影响。为此，叶面喷施纳米营养溶液（NNS）可减轻干旱胁迫的有害影响。
在完全随机设计（CRD）实验中，幼苗在2-3叶期移栽到花盆中，每个花盆装有壤土-堆肥-有机肥料土（3:1:1）。将植株分为两组。（a）对照组（b）施加胁迫组。营养生长阶段的植株，对照组用100%田间持水量（FC）处理，干旱组用60% FC处理，这些水平一直维持到收获。在干旱胁迫处理两周后，在营养生长阶段对所有花盆进行三种浓度（即0%、1%、3%和5%）的NNS处理，间隔5天。
喷施1%的纳米营养溶液可使茎长、茎鲜重和干重、叶片数和花数增加。与对照相比，在喷施3%时，叶片叶绿素、类胡萝卜素和总酚含量最高，而电解质渗漏最低。此外，喷施1%的NNS可提高叶片相对含水量（RWC）%、总可溶性糖和黄酮类化合物含量。在干旱条件下，喷施5%的NNS可使番茄叶片中的总游离氨基酸含量更高，脂质过氧化率最低。抗氧化酶活性呈浓度依赖性增加，在1%、3%和5%时分别逐渐增加。总体而言，本研究为利用纳米营养溶液维持自然资源和确保农业可持续性提供了新的见解。
