Shinkafi Sa'adatu Haruna, Umar Shu'aibu, Neela Vasantha Kumari, Noordin Sabariah Moh'd, Noordin Syafinaz Amin, Hudu Shuaibu Abdullahi, Zainudin Zurina
Department of Medical Microbiology and Parasitology, Usmanu Danfodiyo University Teaching Hospital, Sokoto-Nigeria.
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia.
Afr Health Sci. 2019 Sep;19(3):2378-2389. doi: 10.4314/ahs.v19i3.11.
The term early onset neonatal septicaemia (EONS) refers to invasive bacterial infections that primarily involve the blood stream of neonates during the first 3 days of life. Although early onset neonatal septicaemia is relatively uncommon, it may be associated with case fatality rates of 15-30% and substantial morbidity in surviving infants.
This study describes an unusual septicaemia cases with in neonatal Intensive Care Units.
Bacterial causes of early onset neonatal sepsis in Kuala Lumpur Hospital Malaysia were investigated using broad range 16S rDNA PCR and sequencing. The bacterial DNA was isolated directly from blood without pre-incubation. All samples collected were equally cultured and incubated in automated BACTEC system.
Two hundred and fifty two neonates were recruited in this study with mean (SD) gestational age of 35.9. Neonates with infection lacked microbiological evidence of septicaemia as their blood culture yielded no bacterial growth. However, the PCR analysis of these samples yielded 1100bp corresponding to bacteria species.
This study demonstrates the value of PCR in detecting bacteria where special growth requirement is involved.
早发型新生儿败血症（EONS）一词指的是主要在出生后3天内累及新生儿血流的侵袭性细菌感染。虽然早发型新生儿败血症相对不常见，但它可能与15%-30%的病死率以及存活婴儿的严重发病率相关。
本研究描述了新生儿重症监护病房中不寻常的败血症病例。
使用广谱16S rDNA PCR和测序对马来西亚吉隆坡医院早发型新生儿败血症的细菌病因进行调查。细菌DNA直接从血液中分离，无需预培养。所有采集的样本均在自动BACTEC系统中进行同等培养和孵育。
本研究招募了252名新生儿，平均（标准差）胎龄为35.9。感染的新生儿缺乏败血症的微生物学证据，因为他们的血培养没有细菌生长。然而，这些样本的PCR分析产生了与细菌种类相对应的1100bp。
本研究证明了PCR在检测涉及特殊生长需求的细菌方面的价值。
