NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91191 Gif-sur-Yvette, France.
Analyst. 2020 Apr 7;145(7):2520-2524. doi: 10.1039/d0an00118j. Epub 2020 Mar 4.
High-Resolution Magic-Angle Spinning Chemical Shift Imaging (HR-MAS CSI) has recently been explored for nuclear magnetic resonance (NMR) metabolomics and shows considerable promise in organism research. This is due to its ability to offer a supplemental dimension - spatial metabolic distribution - for profiling. However, HR-MAS CSI suffers from the large centrifugal stress exerted on the sample, which inevitably hinders the metabolic assessment. Herein, a slow sample spinning strategy was implemented and evaluated. The results demonstrate its potential as a highly informative profiling approach for intact specimens, with high quality data and feasibility.
高分辨率魔角旋转化学位移成像（HR-MAS CSI）最近被探索用于核磁共振（NMR）代谢组学，并且在生物体研究中显示出很大的潜力。这是因为它能够提供一个补充的维度 - 空间代谢分布 - 用于分析。然而，HR-MAS CSI 受到施加在样品上的大离心力的影响，这不可避免地阻碍了代谢评估。本文中，实施并评估了一种慢速样品旋转策略。结果表明，它具有作为一种用于完整标本的高度信息丰富的分析方法的潜力，具有高质量的数据和可行性。
