Ito Kengo, Tsuboi Yuuri, Kikuchi Jun
RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
Commun Chem. 2020 Jun 26;3(1):80. doi: 10.1038/s42004-020-0330-1.
Noninvasive evaluation of the spatial distribution of chemical composition and diffusion behavior of materials is becoming possible by advanced nuclear magnetic resonance (NMR) pulse sequence editing. However, there is room for improvement in the spectral resolution and analytical method for application to heterogeneous samples. Here, we develop applications for comprehensively evaluating compounds and their dynamics in intact bodies and heterogeneous systems from NMR data, including spatial z-position, chemical shift, and diffusion or relaxation. This experiment is collectively named spatial molecular-dynamically ordered spectroscopy (SMOOSY). Pseudo-three-dimensional (3D) SMOOSY spectra of an intact shrimp and two heterogeneous systems are recorded to evaluate this methodology. Information about dynamics is mapped onto two-dimensional (2D) chemical shift imaging spectra using a pseudo-spectral imaging method with a processing tool named SMOOSY processor. Pseudo-2D SMOOSY spectral images can non-invasively assess the different dynamics of the compounds at each spatial z-position of the shrimp's body and two heterogeneous systems.
通过先进的核磁共振（NMR）脉冲序列编辑，对材料化学成分的空间分布和扩散行为进行无创评估已成为可能。然而，在应用于异质样品的光谱分辨率和分析方法方面仍有改进空间。在此，我们开发了一些应用程序，用于从NMR数据（包括空间z位置、化学位移以及扩散或弛豫）全面评估完整生物体和异质系统中的化合物及其动力学。该实验被统称为空间分子动力学有序光谱学（SMOOSY）。记录了一只完整虾和两个异质系统的伪三维（3D）SMOOSY光谱，以评估该方法。使用名为SMOOSY处理器的处理工具，通过伪光谱成像方法将动力学信息映射到二维（2D）化学位移成像光谱上。伪二维SMOOSY光谱图像可以无创地评估虾体和两个异质系统每个空间z位置处化合物的不同动力学。
