表面展示的非洲猪瘟病毒（p14.5）可刺激小鼠的免疫反应。
Surface-Displayed ASFV (p14.5) Can Stimulate Immune Responses in Mice.
作者信息
Huang Quntao, Niu Tianming, Zou Boshi, Wang Junhong, Xin Junhong, Niu Hui, Li Nan, Jiang Yuxin, Bao Junfu, Zhang Di, Feng Xize, Sun Tingting, Wang Xin, Yang Kaidian, Wang Ying, Yang Guilian, Zhao Dandan, Wang Chunfeng
机构信息
College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun 130118, China.
出版信息
Vaccines (Basel). 2022 Feb 24;10(3):355. doi: 10.3390/vaccines10030355.
African Swine Fever Virus (ASFV) has spread worldwide, and the lack of vaccines severely negatively impacts the pig industry. In this study, the p14.5 protein encoded by ASFV was used as the antigen, and the p14.5 gene was expressed in vitro using the expression system. Three new functionally recombinant () were constructed and the expressions of the p14.5 protein, p14.5-IL-33-Mus fusion protein and CTA1-p14.5-D-D fusion protein were successfully detected using Western blot analysis. After oral immunization of SPF mice with recombinant , flow cytometry and ELISA were performed to detect the differentiation and maturity of T lymphocytes, B lymphocytes and DCs of the mice, which were higher than those of the control group. Specific antibodies were produced. The immunogenicity of the adjuvant group was stronger than that of the single antigen group, and the IL-33 adjuvant effect was stronger than that of the CTA1-DD adjuvant.
非洲猪瘟病毒（ASFV）已在全球范围内传播，疫苗的缺乏对养猪业产生了严重的负面影响。在本研究中，将ASFV编码的p14.5蛋白用作抗原，并使用表达系统在体外表达p14.5基因。构建了三种新的功能重组体（），并通过蛋白质免疫印迹分析成功检测到了p14.5蛋白、p14.5-IL-33-Mus融合蛋白和CTA1-p14.5-D-D融合蛋白的表达。用重组体对SPF小鼠进行口服免疫后，通过流式细胞术和酶联免疫吸附测定法检测小鼠T淋巴细胞、B淋巴细胞和树突状细胞的分化和成熟情况，其结果高于对照组。产生了特异性抗体。佐剂组的免疫原性强于单抗原组，且IL-33佐剂效果强于CTA1-DD佐剂。
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