Sun Yuqing, Zheng Shaohui, Liu Long, Kong Ying, Zhang Aiwei, Xu Kai, Han Cuiping
School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, People's Republic of China.
Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.
Nanoscale Res Lett. 2020 Mar 4;15(1):55. doi: 10.1186/s11671-020-3288-0.
Doxorubicin entrapped carbon dots (DOX-CDs) were prepared for bioimaging and enhanced intracellular drug delivery. The CDs were synthesized via the hydrothermal method using citrate and urea under 200 °C for 1 h. Then, DOX was successfully conjugated on the CDs via physicochemical interactions. The DOX-CDs exhibited good crystal structure, remarkable aqueous stability, excellent photoluminescence property, and a high quantum yield of 93%. The fluorescent images revealed that the DOX-CDs could be readily taken up by the cancer cells for cell labeling. Furthermore, endo-lysosomal pH-assisted DOX release behavior was observed from DOX-CDs, and the cytotoxicity of DOX-CDs was confirmed by the MTS assay against H0-8910 ovarian cancer cells. In addition, the CDs indicated bright fluorescent signal in the animal imaging test and demonstrated low toxicity after administration for 7 and 21 days. Therefore, the prepared CDs could be a promising imaging probe for biomedical imaging and intracellular drug delivery.
制备了阿霉素包裹的碳点（DOX-CDs）用于生物成像和增强细胞内药物递送。通过水热法，使用柠檬酸盐和尿素在200℃下反应1小时合成碳点。然后，通过物理化学相互作用将阿霉素成功共轭到碳点上。DOX-CDs具有良好的晶体结构、显著的水稳定性、优异的光致发光性能以及93%的高量子产率。荧光图像显示，DOX-CDs能够被癌细胞轻易摄取用于细胞标记。此外，观察到DOX-CDs存在内溶酶体pH辅助的阿霉素释放行为，并且通过针对H0-8910卵巢癌细胞的MTS试验证实了DOX-CDs的细胞毒性。另外，碳点在动物成像试验中显示出明亮的荧光信号，并且在给药7天和21天后显示出低毒性。因此，所制备的碳点可能是一种用于生物医学成像和细胞内药物递送的有前景的成像探针。
