Katargina L A, Osipova N A, Panova A Y, Petrovskaya A V, Nikishina Y O, Murtazina A R, Ugrumov M V
Moscow Helmholtz Institute of Ophthalmology, Ministry of Health of the Russian Federation, 103064, Moscow, Russia.
Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119334, Moscow, Russia.
Dokl Biochem Biophys. 2019 Nov;489(1):373-376. doi: 10.1134/S160767291906005X. Epub 2020 Mar 4.
This work is dedicated to proving our hypothesis that catecholamines and their metabolites play a crucial role in the development of retinopathy of prematurity, which leads to progressive uncontrollable vascularization in the retina, leading to blindness. The study was performed in an animal model of retinopathy of prematurity, which was achieved by hyperoxygenation in rats on postnatal days 7, 14, 21, and 30. The content of catecholamines and their metabolites in the retina of rats was determined by high performance liquid chromatography with electrochemical detection. It was shown that, in the rats with retinopathy, the content of L-DOPA on days 21 and 30 was decreased as compared to the control, whereas the content of noradrenaline on day 14 life increased compared to the control. However, we did not observe changes in the content of dopamine in the experimental animals relative to the control in any period studied. Given the published data on the involvement of catecholamines in the regulation of vasculogenesis in the retina in normal state, our data on the changes in the catecholamine metabolism in the retina in the model of retinopathy of prematurity can be regarded as evidence of the important role of catecholamines in the pathogenesis of this severe disease.
这项工作致力于证明我们的假设，即儿茶酚胺及其代谢产物在早产儿视网膜病变的发展中起关键作用，该病变会导致视网膜中不可控制的血管增生，进而导致失明。该研究在早产儿视网膜病变的动物模型中进行，通过在出生后第7、14、21和30天对大鼠进行高氧处理来实现。大鼠视网膜中儿茶酚胺及其代谢产物的含量通过高效液相色谱电化学检测法测定。结果表明，患有视网膜病变的大鼠在第21天和第30天时，左旋多巴的含量与对照组相比有所降低，而在出生后第14天时，去甲肾上腺素的含量与对照组相比有所增加。然而，在任何研究时期，我们都未观察到实验动物中多巴胺含量相对于对照组有变化。鉴于已发表的关于儿茶酚胺在正常状态下参与视网膜血管生成调节的数据，我们关于早产儿视网膜病变模型中视网膜儿茶酚胺代谢变化的数据可被视为儿茶酚胺在这种严重疾病发病机制中起重要作用的证据。
