甲基 TROSY 光谱学：一种用于研究具有挑战性的生物系统的多功能 NMR 方法。
Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems.
机构信息
Institute of Biophysics and Physical Biochemistry, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
Institute of Biophysics and Physical Biochemistry, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
出版信息
Prog Nucl Magn Reson Spectrosc. 2020 Feb;116:56-84. doi: 10.1016/j.pnmrs.2019.09.004. Epub 2019 Sep 30.
A major goal in structural biology is to unravel how molecular machines function in detail. To that end, solution-state NMR spectroscopy is ideally suited as it is able to study biological assemblies in a near natural environment. Based on methyl TROSY methods, it is now possible to record high-quality data on complexes that are far over 100 kDa in molecular weight. In this review, we discuss the theoretical background of methyl TROSY spectroscopy, the information that can be extracted from methyl TROSY spectra and approaches that can be used to assign methyl resonances in large complexes. In addition, we touch upon insights that have been obtained for a number of challenging biological systems, including the 20S proteasome, the RNA exosome, molecular chaperones and G-protein-coupled receptors. We anticipate that methyl TROSY methods will be increasingly important in modern structural biology approaches, where information regarding static structures is complemented with insights into conformational changes and dynamic intermolecular interactions.
结构生物学的主要目标之一是详细阐明分子机器的功能。为此，溶液核磁共振波谱学是理想的方法，因为它能够在近乎自然的环境中研究生物组装体。基于甲基 TROSY 方法，现在可以记录分子量远远超过 100 kDa 的复合物的高质量数据。在这篇综述中，我们讨论了甲基 TROSY 光谱学的理论背景、可以从甲基 TROSY 光谱中提取的信息以及用于在大型复合物中分配甲基共振的方法。此外，我们还探讨了一些具有挑战性的生物系统的研究进展，包括 20S 蛋白酶体、RNA 外切酶、分子伴侣和 G 蛋白偶联受体。我们预计，在现代结构生物学方法中，甲基 TROSY 方法将变得越来越重要，这些方法可以补充关于构象变化和动态分子间相互作用的信息，从而提供关于静态结构的信息。
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