Fang Qing, Maglangit Fleurdeliz, Mugat Morgane, Urwald Caroline, Kyeremeh Kwaku, Deng Hai
Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland, UK.
Department of Biology and Environmental Science, College of Science, University of the Philippines Cebu, Lahug, Cebu City 6000, Philippines.
Molecules. 2020 Mar 2;25(5):1108. doi: 10.3390/molecules25051108.
Four compounds (-) were isolated from the extracts of sp. CT37 using bioassay in conjunction with mass spectrometric molecular networking (MN) driven isolation. Their complete structures were established by high-resolution electrospray ionization mass spectrometry (HR-ESIMS), and 1D and 2D nuclear magnetic resonance (NMR) data. Legonimide was identified as a new alkaloid containing a rare linear imide motif in its structure, while compounds were already known and their structures were elucidated as 1H-indole-3-carbaldehyde, actinopolymorphol B, ()-1-phenylbutane-2,3-diol, respectively. The biosynthetic pathways of were proposed based on the reported biogenesis of indole alkaloids in literature. Bioactivity tests for and revealed moderate growth inhibition activity against ATCC 10231 with MIC values of 21.54 µg/mL and 11.47 µg/mL, respectively.
采用生物测定结合质谱分子网络（MN）驱动的分离方法，从sp. CT37的提取物中分离出四种化合物（-）。通过高分辨率电喷雾电离质谱（HR-ESIMS）以及一维和二维核磁共振（NMR）数据确定了它们的完整结构。Legonimide被鉴定为一种新的生物碱，其结构中含有罕见的线性酰亚胺基序，而化合物已为人所知，其结构分别被阐明为1H-吲哚-3-甲醛、放线多聚醇B、（）-1-苯基丁烷-2,3-二醇。根据文献中报道的吲哚生物碱生物合成途径，提出了的生物合成途径。对和的生物活性测试显示，它们对ATCC 10231具有中等程度的生长抑制活性，MIC值分别为21.54 µg/mL和11.47 µg/mL。
