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芝麻烘焙不同糖-氨基酸水相模型体系美拉德反应产物的形成及抗氧化活性

Formation and Antioxidant Activity of Maillard Reaction Products Derived from Different Sugar-amino Acid Aqueous Model Systems of Sesame Roasting.

作者信息

Liu Hua-Min, Han Ya-Fei, Wang Nan-Nan, Zheng Yong-Zhan, Wang Xue-De

机构信息

College of Food Science and Technology, Henan University of Technology.

College of Food Science and Technology, Zhengzhou University of Science and Technology.

出版信息

J Oleo Sci. 2020 Apr 3;69(4):391-401. doi: 10.5650/jos.ess19336. Epub 2020 Mar 3.

Abstract

This investigation was carried out to offer insight into the formation and antioxidant activity of Maillard reaction products (MRPs) derived from various sugar-amino acid model systems active in the roasting of sesame seeds. Reducing sugars (glucose, fructose, and xylose) and amino acids (serine, cystine, arginine, and lysine) present in sesame seeds were used to prepare the MRPs at various reaction times, and then the effect of reaction time on the MRPs derived from the various model systems was investigated. Within the first 15 min, the amounts of free amino groups decreased around 40% remaining amino groups of Lys-sugar model and around 75% remaining amino groups of Arg-sugar model. Results indicated that reducing sugar and free amino groups decreased obviously in Lys- and Arg-model systems. Based on correlation coefficient of antioxidant activities assessment and MRP formation in the Lys- and Arg-model systems above 0.978 and an extremely significant correlation in Pearson test exists, a conclusion could be made that these model systems are critical contributing factors in MRP formation during the roasting of sesame seeds. These findings offer insight into the formation and antioxidation of MRPs during the sesame seeds roasting.

摘要

本研究旨在深入了解芝麻烘焙过程中各种糖 - 氨基酸模型体系产生的美拉德反应产物(MRPs)的形成及其抗氧化活性。利用芝麻中存在的还原糖(葡萄糖、果糖和木糖)和氨基酸(丝氨酸、胱氨酸、精氨酸和赖氨酸)在不同反应时间制备MRPs,然后研究反应时间对各种模型体系产生的MRPs的影响。在最初的15分钟内,游离氨基的量减少,赖氨酸 - 糖模型体系中剩余氨基减少约40%,精氨酸 - 糖模型体系中剩余氨基减少约75%。结果表明,在赖氨酸和精氨酸模型体系中还原糖和游离氨基明显减少。基于上述赖氨酸和精氨酸模型体系中抗氧化活性评估与MRP形成的相关系数高于0.978,且在皮尔逊检验中存在极显著相关性,可得出结论:这些模型体系是芝麻烘焙过程中MRP形成的关键因素。这些发现为芝麻烘焙过程中MRPs的形成和抗氧化作用提供了深入了解。

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