Department of Food Science and Technology, The Ohio State University, Columbus, OH, USA.
School of Environment and Natural Resources, The Ohio State University, Columbus, OH, USA.
Sci Rep. 2020 Mar 4;10(1):3998. doi: 10.1038/s41598-020-59691-z.
With increasing levels of fish meal (FM) protein in aquafeeds being replaced with soybean meal (SBM) protein, understanding the molecular mechanisms involved in response to alternative diets has become a critical concern. Thus, the goal of this study was to examine transcriptional differences in the intestine of juvenile yellow perch through RNA-sequencing (RNA-seq), after their initial introduction to a formulated diet with 75% SBM protein inclusion for 61 days, compared to those fed a traditional FM-based diet. Transcriptomic analysis revealed a concise set of differentially expressed genes in juveniles fed the SBM-based diet, the majority of which were intrinsic to the cholesterol biosynthesis pathway. Analysis of total body lipid and cholesterol levels were also investigated, with no between-treatment differences detected. Results of this study demonstrate that in response to SBM-based diets, yellow perch juveniles up-regulate the cholesterol biosynthesis pathway in order to maintain homeostasis. These findings suggest that the upregulation of the cholesterol biosynthesis pathway may negatively impact fish growth due to its large energy expenditure, and future studies are warranted.
随着水产饲料中鱼粉（FM）蛋白水平的不断提高，用大豆粉（SBM）蛋白替代，了解替代饮食的反应中涉及的分子机制已成为一个关键问题。因此，本研究的目的是通过 RNA 测序（RNA-seq），在幼黄鲈最初用含 75%SBM 蛋白的配方饲料喂养 61 天后，检测其肠道的转录差异，与那些用传统的基于 FM 的饮食喂养的相比。转录组分析显示，在喂食基于 SBM 的饮食的幼鱼中，有一组数量不多的差异表达基因，其中大多数与胆固醇生物合成途径有关。还对总体脂和胆固醇水平进行了分析，未发现处理间差异。本研究的结果表明，为了维持体内平衡，幼黄鲈对基于 SBM 的饮食作出反应，上调了胆固醇生物合成途径。这些发现表明，胆固醇生物合成途径的上调可能由于其巨大的能量消耗而对鱼类生长产生负面影响，因此需要进一步研究。
