Piergiacomi G, Giulietti A A, Muti S, Silveri F, Cervini C
Department of Rheumatology, School of Medicine, University of Ancona, Italy.
Z Rheumatol. 1988 May-Jun;47(3):151-5.
The aim of the study was to investigate the interaction of opsonized zymosan with polymorphonuclear leukocytes (PMNLs) from 26 rheumatoid arthritis patients (six males and 20 females; mean age, 51.88 +/- 11.15 years; range, 33-72 years) and 11 control subjects (two males and nine females; mean age, 56.54 +/- 12.1 years; range, 33-75 years) using a luminol-amplified chemiluminescence (CL). The data obtained suggest that the opsonized zymosan stimulated CL is decreased in untreated rheumatoid patients compared with control subjects. The decreased stimulated CL may be the result of a physico-chemical alteration of the membrane (including decreased membrane fluidity and translocation of receptor sites) induced by free radicals of oxygen and/or by multiple exposures to chemotaxins. Auranofin enhances stimulated CL although the mechanism is not clear. Auranofin could interact with membrane lipids, induce changes in membrane surface charges and increase membrane fluidity. Our results are difficult to compare with those of previous studies, since different assays, and chemoattractants were applied. In addition, the disease activity and medication were also different.
本研究的目的是使用鲁米诺增强化学发光法（CL），研究调理酵母聚糖与26例类风湿性关节炎患者（6例男性和20例女性；平均年龄51.88±11.15岁；范围33 - 72岁）及11名对照受试者（2例男性和9例女性；平均年龄56.54±12.1岁；范围33 - 75岁）的多形核白细胞（PMNLs）之间的相互作用。所获得的数据表明，与对照受试者相比，未经治疗的类风湿患者中调理酵母聚糖刺激的CL降低。刺激的CL降低可能是由氧自由基和/或多次暴露于趋化因子引起的膜物理化学改变（包括膜流动性降低和受体位点易位）的结果。金诺芬可增强刺激的CL，尽管其机制尚不清楚。金诺芬可能与膜脂质相互作用，引起膜表面电荷变化并增加膜流动性。我们的结果难以与先前研究的结果进行比较，因为采用了不同的检测方法和趋化剂。此外，疾病活动度和用药情况也不同。
