James D W, Betts W H, Cleland L G
J Rheumatol. 1983 Apr;10(2):184-9.
Chemiluminescence (CL) was used to measure the oxidative burst of polymorphonuclear leukocytes (PMNL) from peripheral blood (PB) and synovial fluid (SF) of patients with inflammatory arthritis. The basal CL of SFPMNL was greater than that of PBPMNL. Opsonized zymosan, N-formyl methionyl-leucyl-phenylalanine (FMLP), cell-free inflammatory SF and heat aggregated human IgG (HAIG) all stimulated PMNL CL but the response of SFPMNL to HAIG or to reexposure to SF was greater than that of PBPMNL. This enhanced responsiveness of SFPMNL to HAIG could be induced in PBPMNL by preincubation with chemotactic concentrations of FMLP. These studies suggest a mechanism by which PMNL migrating to sites of inflammation may become sensitized to a subsequent stimulation by soluble immune complexes at the inflammatory site.
采用化学发光法检测炎症性关节炎患者外周血（PB）和滑液（SF）中多形核白细胞（PMNL）的氧化爆发。滑液多形核白细胞（SFPMNL）的基础化学发光高于外周血多形核白细胞（PBPMNL）。调理酵母聚糖、N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）、无细胞炎症性滑液和热聚集人IgG（HAIG）均可刺激多形核白细胞化学发光，但滑液多形核白细胞对HAIG或再次暴露于滑液的反应大于外周血多形核白细胞。通过趋化浓度的FMLP预孵育，可使外周血多形核白细胞对外源性HAIG的反应性增强。这些研究提示了一种机制，即迁移至炎症部位的多形核白细胞可能对炎症部位可溶性免疫复合物的后续刺激产生致敏作用。
