Cumming Royce T, Bank Sarah, Le Tirant Stephane, Bradler Sven
Associate Researcher, Montréal Insectarium, 4581 rue Sherbrooke est, Montréal, Québec, Canada, H1X 2B2.
Richard Gilder Graduate School, American Museum of Natural History, New York, NY 10024, USA.
Zookeys. 2020 Feb 19;913:89-126. doi: 10.3897/zookeys.913.49044. eCollection 2020.
Within the last two years, the leaf insects of the genus of both the islands of Java and Sumatra have been reviewed extensively based on morphological observations. However, cryptic species which cannot be differentiated morphologically may be present among the various populations. Since it has frequently been demonstrated that analyses based on molecular data can bring clarity in such cases, we conducted a phylogenetic analysis based on three genes (nuclear gene 28S and mitochondrial genes COI and 16S) from the species of these islands. The results show distinct molecular divergence for several populations and suggest the presence of two new cryptic species, morphologically inseparable from Brock, 1999. From Sumatra, the population originally thought to be a range expansion for , is now here described as , with the only consistent morphological difference being the color of the eggs between the two populations (dark brown in and tan in ). Further, an additional population with purple coxae from Java was morphologically examined and found to have no consistent features to separate it morphologically from the other purple coxae species. This cryptic species from Java was however shown to be molecularly distinct from the other purple coxae populations from Sumatra and Peninsular Malaysia and is here described as In addition, is here officially reported from Java for the first time based on both historic and modern records of male specimens.
在过去两年里，基于形态学观察，对爪哇岛和苏门答腊岛的叶螽属昆虫进行了广泛的研究。然而，不同种群中可能存在形态上无法区分的隐存种。由于基于分子数据的分析在这类情况下常常能带来清晰的结果，我们基于来自这些岛屿的叶螽物种的三个基因（核基因28S以及线粒体基因COI和16S）进行了系统发育分析。结果显示几个种群存在明显的分子差异，并表明存在两个新的隐存种，它们在形态上与1999年的布罗克叶螽无法区分。来自苏门答腊岛的种群，最初被认为是布罗克叶螽的分布范围扩展，现在被描述为新种，这两个种群之间唯一一致的形态差异是卵的颜色（新种的卵为深棕色，布罗克叶螽的卵为棕褐色）。此外，对来自爪哇岛的一个具有紫色基节的额外种群进行了形态学检查，发现它在形态上没有与其他具有紫色基节的物种区分开来的一致特征。然而，这个来自爪哇岛的隐存种在分子上与来自苏门答腊岛和马来西亚半岛的其他具有紫色基节的种群不同，在此被描述为新种。此外，根据雄性标本的历史和现代记录，新种首次在爪哇岛被正式报道。
