Santana Thalita, Nagata Gabriela, Saturno Juvani Lago, Trierveiler Marília
Oral and Maxillofacial Pathology Department, School of Dentistry, University of São Paulo - USP, São Paulo, Brazil.
School of Dentistry, Hermínio Ometto University Center, UNIARARAS, Araras, Brazil.
J Cutan Pathol. 2020 Jul;47(7):592-600. doi: 10.1111/cup.13677. Epub 2020 Mar 20.
Actinic cheilitis is induced by chronic exposure to ultraviolet radiation and shows solar elastosis, a feature that has been associated with mast cell infiltrates. This study aimed to investigate the area of solar elastosis, collagen loss, and mast cell infiltrates in a series of actinic cheilitis.
We evaluated the epithelial dysplasia in 52 cases of actinic cheilitis and the solar elastosis with Weigert's resorcin-fuchsin. Collagen loss was evaluated with Picrosirius red, analyzed under polarized microscopy, and scored from 1 to 3. Elastosis proportionate area (EPA) was calculated with image software. Mast cells were highlighted with toluidine blue stain.
EPA varied from 2% to 45%, with a mean of 17.1% in the cases, with no differences among epithelial dysplasia degrees. Most cases presented collagen loss scores of 2 or 3, and higher loss of type I collagen was associated with older age. Mast cell density was higher in severe epithelial dysplasia (P = 0.002) and in high-risk cases (P = 0.01).
Actinic cheilitis presented variable EPA and marked loss of type I collagen; however, these features were not associated with the degrees of epithelial dysplasia. Besides, mast cell density increased with epithelial dysplasia worsening and this was not associated with elastosis area or collagen loss.
光化性唇炎由长期暴露于紫外线辐射引起，表现为日光性弹力组织变性，这一特征与肥大细胞浸润有关。本研究旨在调查一系列光化性唇炎中日光性弹力组织变性的面积、胶原蛋白损失及肥大细胞浸润情况。
我们评估了52例光化性唇炎的上皮发育异常情况，并使用魏格特氏间苯二酚品红对日光性弹力组织变性进行评估。使用苦味酸天狼星红评估胶原蛋白损失，在偏光显微镜下进行分析，并从1到3进行评分。使用图像软件计算弹力组织变性比例面积（EPA）。用甲苯胺蓝染色突出显示肥大细胞。
EPA在2%至45%之间变化，病例的平均值为17.1%，上皮发育异常程度之间无差异。大多数病例的胶原蛋白损失评分为2或3，I型胶原蛋白的更高损失与年龄较大有关。严重上皮发育异常（P = 0.002）和高危病例（P = 0.01）中的肥大细胞密度更高。
光化性唇炎呈现出可变的EPA和明显的I型胶原蛋白损失；然而，这些特征与上皮发育异常程度无关。此外，肥大细胞密度随着上皮发育异常的恶化而增加，且这与弹力组织变性面积或胶原蛋白损失无关。
