Pharmacology and Toxicology Department, College of Pharmacy, King Saud University, Riyadh, KSA.
Common First Year, King Saud University, Riyadh, KSA.
J Biochem Mol Toxicol. 2020 Jun;34(6):e22481. doi: 10.1002/jbt.22481. Epub 2020 Mar 5.
The present work was aimed to evaluate the protective effects of alpha-tocopherol (α-toco) and/or Lactobacillus plantarum (LCB) against testicular atrophy induced by mercuric chloride (MCH). Rats were injected with 5 mg/kg MCH for 5 days consecutively, then treated with 100 mg/kg α-toco and 6 × 10 CFU 1.8701/kg LCB alone or together for 3 weeks. The MCH elevated serum TNF-α, IL- 6, caspase-3, and testicular malondialdehyde. However, serum testosterone, dehydroepiandrosterone, testicular messenger RNA of a steroidogenic acute regulatory protein, 17-β-hydroxysteroid dehydrogenase, 3β-hydroxysteroid dehydrogenase, glutathione level, and superoxide dismutase activity were decreased. Protein expression of Nrf2 was downregulated whereas that of Bax and DNA fragmentation was upregulated in the testicular tissues. Treatment with α-toco and LCB ameliorated the deviated biochemical parameters and improved tissue injury. It was concluded that the combination of LCB and α-toco achieved promising results in the amelioration of MCH-induced testicular atrophy. Nrf2, Bax expressions, and DNA fragmentation are involved in the testicular atrophy induced by MCH.
本研究旨在评估α-生育酚（α-toco）和/或植物乳杆菌（LCB）对氯化汞（MCH）诱导的睾丸萎缩的保护作用。大鼠连续 5 天注射 5mg/kg MCH，然后用 100mg/kg α-toco 和 6×10 CFU 1.8701/kg LCB 单独或联合治疗 3 周。MCH 升高了血清 TNF-α、IL-6、caspase-3 和睾丸丙二醛。然而，血清睾酮、脱氢表雄酮、类固醇生成急性调节蛋白的睾丸信使 RNA、17-β-羟类固醇脱氢酶、3β-羟类固醇脱氢酶、谷胱甘肽水平和超氧化物歧化酶活性降低。睾丸组织中 Nrf2 的蛋白表达下调，而 Bax 和 DNA 片段化的蛋白表达上调。α-toco 和 LCB 的治疗改善了偏离的生化参数并改善了组织损伤。综上所述，LCB 和 α-toco 的联合应用在改善 MCH 诱导的睾丸萎缩方面取得了显著效果。Nrf2、Bax 表达和 DNA 片段化参与了 MCH 诱导的睾丸萎缩。
