Suzuki Rio, Konno Hiroyuki
Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Yamagata 992-8510, Japan.
Org Lett. 2020 May 1;22(9):3309-3312. doi: 10.1021/acs.orglett.0c00445. Epub 2020 Mar 5.
A novel and reversible detection method for N-terminal amino groups and alternative to the Kaiser test during Fmoc-SPPS has been developed. The utility of the new method was exemplified in its monitoring of the construction of a peptide, the final yield of which was higher than that of an identical peptide constructed under identical conditions but using the Kaiser test, and its scope was established to include all 20 proteinogenic amino acids as well as nonproteinogenic ones.
已开发出一种用于检测N端氨基的新型可逆检测方法，可在Fmoc固相肽合成过程中替代凯氏测试。该新方法的实用性体现在其对肽构建过程的监测中，所合成肽的最终产率高于在相同条件下使用凯氏测试构建的相同肽，并且其适用范围确定为包括所有20种蛋白质ogenic氨基酸以及非蛋白质ogenic氨基酸。
