Chair for Biological Imaging, Center for Translational Cancer Research (TranslaTUM), Technical University of Munich, Munich, Germany.
Helmholtz Zentrum München, Institute of Biological and Medical Imaging, Neuherberg, Germany.
J Biophotonics. 2020 Jun;13(6):e201960169. doi: 10.1002/jbio.201960169. Epub 2020 Mar 25.
Perfusion and oxygenation are critical parameters of muscle metabolism in health and disease. They have been both the target of many studies, in particular using near-infrared spectroscopy (NIRS). However, difficulties with quantifying NIRS signals have limited a wide dissemination of the method to the clinics. Our aim was to investigate whether clinical multispectral optoacoustic tomography (MSOT) could enable the label-free imaging of muscle perfusion and oxygenation under clinically relevant challenges: the arterial and venous occlusion. We employed a hybrid clinical MSOT/ultrasound system equipped with a hand-held scanning probe to visualize hemodynamic and oxygenation changes in skeletal muscle under arterial and venous occlusions. Four (N = 4) healthy volunteers were scanned over the forearm for both 3-minute occlusion challenges. MSOT-recorded pathophysiologically expected results during tests of disturbed blood flow with high resolution and without the need for contrast agents. During arterial occlusion, MSOT-extracted Hb-values showed an increase, while HbO - and total blood volume (TBV)-values remained roughly steady, followed by a discrete increase during the hyperemic period after cuff deflation. During venous occlusion, results showed a clear increase in intramuscular HbO , Hb and TBV within the segmented muscle area. MSOT was found to be capable of label-free non-invasive imaging of muscle hemodynamics and oxygenation under arterial and venous occlusion. We introduce herein MSOT as a novel modality for the assessment of vascular disorders characterized by disturbed blood flow, such as acute limb ischemia and venous thrombosis.
灌注和氧合是健康和疾病中肌肉代谢的关键参数。它们一直是许多研究的目标，特别是使用近红外光谱（NIRS）。然而，量化 NIRS 信号的困难限制了该方法在临床上的广泛应用。我们的目的是研究临床多光谱光声断层扫描（MSOT）是否能够在临床上相关的挑战下（动脉和静脉阻塞）实现对肌肉灌注和氧合的无标记成像。我们使用配备手持式扫描探头的混合临床 MSOT/超声系统来可视化骨骼肌在动脉和静脉阻塞下的血液动力学和氧合变化。四名（N = 4）健康志愿者在前臂上进行了 3 分钟的阻塞挑战扫描。MSOT 以高分辨率记录了在血流紊乱测试期间的病理生理学预期结果，而无需使用造影剂。在动脉阻塞期间，MSOT 提取的 Hb 值增加，而 HbO 和总血液量（TBV）值基本保持稳定，随后在袖口放气后的充血期出现离散增加。在静脉阻塞期间，结果显示在分段肌肉区域内，肌肉内 HbO 、Hb 和 TBV 明显增加。MSOT 被发现能够在动脉和静脉阻塞下对肌肉血液动力学和氧合进行无标记的非侵入性成像。我们在此介绍 MSOT 作为一种评估以血流紊乱为特征的血管疾病的新方法，例如急性肢体缺血和静脉血栓形成。
