Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Robert-Koch-Straße 4, 35037 Marburg, Germany.
State Key Laboratory of Mycology and CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Org Lett. 2020 Mar 20;22(6):2256-2260. doi: 10.1021/acs.orglett.0c00440. Epub 2020 Mar 5.
The biosynthetic pathway of the prenylated salicylaldehyde flavoglaucin and congeners in was elucidated by genome mining, heterologous expression, precursor feeding, and biochemical characterization. The polyketide skeleton was released as alkylated salicyl alcohols, which is a prerequisite for consecutive hydroxylation and prenylation, before reoxidation to the final aldehyde products. Our results provide an excellent example for a highly programmed machinery in natural product biosynthesis.
通过基因组挖掘、异源表达、前体喂养和生化特征分析，阐明了 中prenylated salicylaldehyde flavoglaucin 和同系物的生物合成途径。多酮骨架以烷基化水杨醇的形式释放，这是连续羟化和prenylation 的前提，然后再氧化为最终的醛产物。我们的结果为天然产物生物合成中高度程序化的机制提供了一个极好的范例。
