State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Sci Adv. 2022 Apr 29;8(17):eabo6094. doi: 10.1126/sciadv.abo6094. Epub 2022 Apr 27.
Microbial communication has attracted notable attention as an indicator of microbial interactions that lead to marked alterations of secondary metabolites (SMs) in varied environments. However, the mechanisms responsible for SM regulation are not fully understood, especially in fungal-fungal interactions. Here, cocultivation of an endophytic fungus with the model fungus and several other filamentous fungi triggered widespread alteration of SMs. Multiple silent biosynthetic gene clusters in were activated by transcriptome and metabolome analysis. Unprecedentedly, gene deletion and replacement proved that a partial loss-of-function VeA1 protein, but not VeA, was associated with the widespread SM changes in both and during cocultivation. VeA1 regulation required the transcription factor SclB and the velvet complex members LaeA and VelB for producing aspernidines as representative formation of SMs in . This study provides new insights into the mechanism that trigger metabolic changes during fungal-fungal interactions.
微生物通讯作为微生物相互作用的指标引起了人们的关注，这些相互作用导致次级代谢产物（SMs）在不同环境中发生明显改变。然而，负责调节 SM 的机制尚不完全清楚，特别是在真菌-真菌相互作用中。在这里，通过与模式真菌 和其他几种丝状真菌共培养，内生真菌 触发了 SMs 的广泛改变。通过转录组和代谢组分析， 中的多个沉默生物合成基因簇被激活。史无前例的是，基因缺失和替换证明部分丧失功能的 VeA1 蛋白，而不是 VeA，与 和 在共培养过程中的广泛 SM 变化有关。VeA1 的调控需要转录因子 SclB 和 velvet 复合物成员 LaeA 和 VelB 来产生 aspernidines，作为 SMs 的代表性形成 在 中。这项研究为触发真菌-真菌相互作用过程中代谢变化的机制提供了新的见解。
