Interdisciplinary Program of Bioenergy and Biomaterials Graduate School, College of Engineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
Department of Optometry, College of Energy and Biotechnology, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea; Convergence Institute of Biomaterials and Bioengineering, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea.
J Hazard Mater. 2020 Jul 5;393:122400. doi: 10.1016/j.jhazmat.2020.122400. Epub 2020 Feb 25.
Environmental pollution by anthropogenic chemicals has become a considerable problem. Organochlorine pesticides (OCPs), a subclass of persistent organic pollutants, are used as insecticides and industrial chemicals. They are lipophilic and minimally degradable, and they easily accumulate in the environment and human body. Epidemiological studies have demonstrated that exposure to OCPs strongly correlates with the development of type 2 diabetes, which involves mitochondrial dysfunction. To clarify their effects, OCP mixtures (β-hexachlorocyclohexane, heptachlor, hexachlorobenzene, 4,4'-DDT, and chlordane) were used to treat mitochondria from zebrafish livers. Results showed that as OCP concentrations increased, Ca intake into the mitochondria rose, which increased the activity of mitochondrial complexes I, II, IV, and citrate synthase. Complex III yielded the opposite result because the OCP mixture mimicked decylubiquinol, a natural substrate of complex III. Our results reflect the actual state of toxins, non-monotonic, in the environment, which is important for determining the consequences of OCPs on mitochondrial dysfunction.
人为化学物质造成的环境污染已成为一个相当大的问题。有机氯农药（OCPs）是持久性有机污染物的一个子类，被用作杀虫剂和工业化学品。它们具有亲脂性和最小的可降解性，容易在环境和人体中积累。流行病学研究表明，接触 OCPs 与 2 型糖尿病的发展密切相关，这涉及到线粒体功能障碍。为了阐明它们的作用，使用 OCP 混合物（β-六氯环己烷、七氯、六氯苯、4,4'-滴滴涕和氯丹）处理斑马鱼肝线粒体。结果表明，随着 OCP 浓度的增加，线粒体摄取 Ca2+的量增加，这增加了线粒体复合物 I、II、IV 和柠檬酸合酶的活性。复合物 III 产生了相反的结果，因为 OCP 混合物模拟了复合物 III 的天然底物癸基泛醌。我们的结果反映了环境中实际存在的毒素状态，这种状态对于确定 OCPs 对线粒体功能障碍的后果非常重要。
