Juhlin C, Holmdahl R, Johansson H, Rastad J, Akerström G, Klareskog L
Proc Natl Acad Sci U S A. 1987 May;84(9):2990-4. doi: 10.1073/pnas.84.9.2990.
Four monoclonal anti-parathyroid antibodies were generated after immunization of mice with intact cells from human parathyroid tissue. All four monoclonal antibodies reacted in immunohistochemistry with structures present on parathyroid epithelial cells and proximal-tubule cells of the kidney but were unreactive with all other human tissues investigated. Immunofluorescence microscopy on suspended human parathyroid cells showed that the antibodies reacted with structures present on the cell surface. Two of these antibodies efficiently blocked the increase in cytoplasmic calcium concentration of parathyroid cells that is normally associated with increased concentrations of extracellular calcium. The results indicate that these two antibodies interfere with a calcium-sensing mechanism of parathyroid cells--i.e., a potential calcium receptor by which extracellular calcium regulates cytoplasmic calcium and hormone release in these cells.
用人甲状旁腺组织的完整细胞免疫小鼠后，产生了四种单克隆抗甲状旁腺抗体。所有这四种单克隆抗体在免疫组织化学中均与甲状旁腺上皮细胞和肾近端小管细胞上的结构发生反应，但与所研究的所有其他人体组织均无反应。对悬浮的人甲状旁腺细胞进行免疫荧光显微镜检查显示，这些抗体与细胞表面的结构发生反应。其中两种抗体有效地阻断了甲状旁腺细胞胞质钙浓度的升高，而这种升高通常与细胞外钙浓度的升高有关。结果表明，这两种抗体干扰了甲状旁腺细胞的钙传感机制，即一种潜在的钙受体，通过该受体细胞外钙调节这些细胞中的胞质钙和激素释放。
