Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.
Eur J Clin Microbiol Infect Dis. 2020 Jul;39(7):1391-1399. doi: 10.1007/s10096-020-03857-0. Epub 2020 Mar 5.
This study aims to investigate the antimicrobial and antibiofilm activity of berberine chloride (BBR) and vancomycin (VAN) as well as synergistic combinations of BBR with VAN against Clostridioides difficile strains. The effect of different concentrations of BBR on strain motility was also assessed. Twelve C. difficile strains (two reference C. difficile 630, ATCC 9689, and one control M120, and 9 clinical C. difficile strains belonging to the PCR-ribotype (RT027)) were collected and investigated for their susceptibility to BBR and VAN in planktonic and biofilm forms. Both the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of BBR for the C. difficile strains were found to vary over a broad range (256-1.024 mg/L and 256-16.384 mg/L, respectively). The MIC and MBC of VAN also varied greatly, ranging from 0.25 to 4.0 mg/L for MIC and 0.25 to 64.0 mg/L for MBC. The synergistic effect of the sub-MIC (1/2 MIC) BBR with VAN reduced of MICs of VAN against the planktonic forms of ten C. difficile strains. The sub-MIC of BBR enhanced the biofilm formation of one strain and was found to be statistically significant. In addition, the sub-MIC of BBR with VAN surprisingly enhanced the biofilm formation of one C. difficile strain. The effect of inhibition of motility in the presence of BBR was statistically significant for 3 clinical strains (p < 0.05). Altogether, BBR exhibited strong antimicrobial activity against C. difficile, and the analysis of the combination of BBR with VAN showed a synergistic effect.
本研究旨在探究盐酸小檗碱（BBR）和万古霉素（VAN）的抗菌和抗生物膜活性，以及 BBR 与 VAN 的协同组合对艰难梭菌菌株的作用。还评估了不同浓度的 BBR 对菌株运动性的影响。收集了 12 株艰难梭菌（2 株参考艰难梭菌 630、ATCC 9689 和 1 株对照 M120，以及 9 株属于 PCR-核糖体分型（RT027）的临床艰难梭菌菌株），并研究了它们在浮游和生物膜形式下对 BBR 和 VAN 的敏感性。艰难梭菌菌株的 BBR 的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）变化范围很广（分别为 256-1.024mg/L 和 256-16.384mg/L）。VAN 的 MIC 和 MBC 也有很大差异，MIC 范围为 0.25 至 4.0mg/L，MBC 范围为 0.25 至 64.0mg/L。亚 MIC（1/2 MIC）BBR 与 VAN 的协同作用降低了 10 株艰难梭菌浮游形式对 VAN 的 MIC。亚 MIC 的 BBR 增强了一株菌株的生物膜形成，且具有统计学意义。此外，亚 MIC 的 BBR 与 VAN 出人意料地增强了一株艰难梭菌的生物膜形成。BBR 存在时对 3 株临床菌株的运动性抑制作用具有统计学意义（p<0.05）。总的来说，BBR 对艰难梭菌表现出强大的抗菌活性，BBR 与 VAN 的联合分析显示出协同作用。
