Department of Medical Microbiology, Medical University of Warsaw, 02-004 Warsaw, Poland.
Imaging Laboratory, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
Molecules. 2022 Oct 30;27(21):7385. doi: 10.3390/molecules27217385.
There is an ongoing search for alternative treatments for infections. The aim of the study was to investigate the antibacterial and antibiotic activity of bee products against strains with different polymerase chain reaction ribotypes (RTs). The minimum inhibitory concentration (MICs) of Manuka honey 550+, goldenrod honey, pine honey, and bee bread were determined by the broth dilution method. adhesion to HT-29, HT-29 MTX, and CCD 841 CoN cell lines was assessed. Biofilm was cultured in titration plates and visualized by confocal microscopy. The MICs of Manuka honey for 630 and ATCC 9689 strains and control strain, M 120, were 6.25%, 6.25%, and 1.56% (/), respectively; of goldenrod honey, 50%, 50%, and 12.5%, respectively; of pine honey, 25%, 25%, and 25%, respectively; and of bee bread, 100 mg/L, 50 mg/L, and 100 mg/L, respectively. Manuka honey (1%) increased adhesion of RT176 strains, and one strain of RT023, to the CCD 841 cell line. Pine honey (1%) increased RT027 adhesion to the HT-29 cell line. Manuka honey, pine honey, and bee bread at subinhibitory concentrations increased the adhesion of . Our research proved that bee products are active against the tested strains of
目前正在寻找替代治疗感染的方法。本研究旨在研究蜂产品对具有不同聚合酶链反应核糖型（RT）的菌株的抗菌和抗生素活性。采用肉汤稀释法测定麦卢卡蜂蜜 550+、款冬蜂蜜、松树蜂蜜和蜂粮的最小抑菌浓度（MIC）。评估了对 HT-29、HT-29 MTX 和 CCD 841 CoN 细胞系的粘附作用。在滴定板中培养生物膜，并通过共聚焦显微镜进行可视化。麦卢卡蜂蜜对 630 和 ATCC 9689 菌株和对照菌株 M 120 的 MIC 分别为 6.25%、6.25%和 1.56%（/）；款冬蜂蜜分别为 50%、50%和 12.5%；松树蜂蜜分别为 25%、25%和 25%；蜂粮分别为 100 mg/L、50 mg/L 和 100 mg/L。麦卢卡蜂蜜（1%）增加了 RT176 菌株和一种 RT023 菌株对 CCD 841 细胞系的粘附。松树蜂蜜（1%）增加了 RT027 对 HT-29 细胞系的粘附。在亚抑菌浓度下，麦卢卡蜂蜜、松树蜂蜜和蜂粮增加了 的粘附作用。我们的研究证明了蜂产品对测试菌株具有活性。
