几种用于从污染水体中分离嗜水气单胞菌的选择性培养基的评估。
Evaluation of several selective media for recovery of Aeromonas hydrophila from polluted waters.
作者信息
Arcos M L, de Vicente A, Moriñigo M A, Romero P, Borrego J J
机构信息
Departamento de Microbiología, Facultad de Ciencias, Universidad de Malaga, Spain.
出版信息
Appl Environ Microbiol. 1988 Nov;54(11):2786-92. doi: 10.1128/aem.54.11.2786-2792.1988.
Eleven media were studied for their suitability in the selective isolation of Aeromonas hydrophila. Preliminary results showed that five of them (inositol-brilliant green-bile salts agar, bile salts-brilliant green agar, Rimler-Shotts agar, xylose-sodium deoxycholate-citrate agar, and dextrin-fuchsin-sulfite agar) allowed the growth of several microorganisms that are usually present in the same samples in which A. hydrophila is found. Six media (mA agar, modified Rimler-Shotts agar, DNase-toluidine blue-ampicillin agar, Pril-xylose-ampicillin agar, MacConkey-trehalose agar, and starch-bile salts agar) were selected for evaluation as recovery selective media on the basis of their efficiency in the isolation of A. hydrophila from natural water samples. mA agar showed the best recovery rate and also an acceptable specificity, but its selectivity was low. Another medium that can be considered is DNase-toluidine blue-ampicillin agar, which showed good accuracy, but its specificity was low.
对11种培养基进行了研究，以评估它们在选择性分离嗜水气单胞菌方面的适用性。初步结果表明，其中5种培养基（肌醇-煌绿-胆盐琼脂、胆盐-煌绿琼脂、里姆勒-肖茨琼脂、木糖-脱氧胆酸钠-柠檬酸盐琼脂和糊精-品红-亚硫酸盐琼脂）允许几种通常与嗜水气单胞菌存在于相同样本中的微生物生长。基于它们从天然水样中分离嗜水气单胞菌的效率，选择了6种培养基（mA琼脂、改良里姆勒-肖茨琼脂、DNase-甲苯胺蓝-氨苄青霉素琼脂、普里尔-木糖-氨苄青霉素琼脂、麦康凯-海藻糖琼脂和淀粉-胆盐琼脂）作为恢复选择性培养基进行评估。mA琼脂显示出最佳的回收率，特异性也可以接受，但选择性较低。另一种可以考虑的培养基是DNase-甲苯胺蓝-氨苄青霉素琼脂，其准确性良好，但特异性较低。
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