Bernagozzi M, Bianucci F, Scerre E, Sacchetti R
Università degli Studi di Bologna Facoltà di Medicina e Chirurgia II, Cattedra di Igiene.
Zentralbl Hyg Umweltmed. 1994 Jan;195(2):121-34.
A comparison was made of three different growing media already proposed by some authors for the recovery of Aeromonas hydrophila in environmental water samples of various origins. The media tested were Rippey and Cabelli m Aeromonas agar, Rimler-Shotts agar and the Ryan Aeromonas Base Medium with the addition of ampicillin. The efficiency of the three media was evaluated on the basis of the following reference criteria: accuracy, selectivity and specificity. The results obtained, analyzed by calculating the Kendall concordance coefficients, demonstrated that m Aeromonas agar is the most suitable media for quantitative recovery of Aeromonas hydrophila from surface water samples even if a reduction in the total heterotrophic load of 3-logs was never obtained with this medium.
对一些作者已经提出的三种不同生长培养基进行了比较,用于从各种来源的环境水样中分离嗜水气单胞菌。测试的培养基有Rippey和Cabelli嗜水气单胞菌琼脂、Rimler-Shotts琼脂以及添加了氨苄青霉素的Ryan嗜水气单胞菌基础培养基。根据以下参考标准评估了这三种培养基的效率:准确性、选择性和特异性。通过计算肯德尔和谐系数分析得到的结果表明,嗜水气单胞菌琼脂是从地表水样品中定量分离嗜水气单胞菌的最合适培养基,即使使用该培养基从未使总异养菌负荷降低3个对数。