Department of Food Sciences & Technology, BOKU-University of Natural Resources & Life Sciences, Vienna, Austria.
Doctoral Program BioToP, Biomolecular Technology of Proteins, BOKU-University of Natural Resources & Life Sciences Vienna, Vienna, Austria.
Biotechniques. 2020 May;68(5):279-282. doi: 10.2144/btn-2019-0152. Epub 2020 Mar 6.
Species of the genus are filamentous fungi commonly used in research, industry and agriculture. strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as might be exploited as biocontrol agents. A careful preparation of inocula, which consists mainly of conidia (asexual spores), is of immense importance. Conidia concentration is still mostly determined with the help of a hemocytometer; however, as a more accurate and time-saving alternative, absorbance can be used to estimate fungal spore counts. We established a spectrophotometric method for fast and reliable preparation of inocula by evaluating the effect of size, shape and pigmentation of the conidia at different wavelengths.
属的物种是在研究、工业和农业中常用的丝状真菌。 菌株是纤维素酶和半纤维素酶的主要生产者，也是表达宿主；其他一些物种，如 可能被用作生物防治剂。 精心制备接种物非常重要，接种物主要由分生孢子（无性孢子）组成。 分生孢子浓度仍然主要通过血球计数器来确定；但是，作为更准确和节省时间的替代方法，可以使用吸光度来估计真菌孢子的数量。 我们通过评估不同波长下分生孢子的大小、形状和色素沉着对吸光度的影响，建立了一种分光光度法来快速可靠地制备接种物。
